Insights into the conformational changes of several human lysozyme variants associated with hereditary systemic amyloidosis

Hsuan Liang Liu, Yi Ching Wu, Jian Hua Zhao, Yi Fan Liu, Chi Hung Huang, Hsu Wei Fang, Yih Ho

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, various ethanol- and temperature-induced molecular dynamics simulations were conducted to investigate the conformational changes of several human lysozyme variants (I56T, D67H, and T70N) associated with hereditary systemic amyloidosis. The results show that these variants are all more sensitive to conditions affecting the structural integrity of this protein. The structural analyses of these variants reveal a high population of more unstable β-domain and distorted hydrophobic core compared to the wild-type human lysozyme, particularly for the two natural amyloidogenic variants D67H and I56T. For the D67H variant, the distance between the mass centers of residues 54 and 67 was found to elongate as a result of the destruction of the hydrogen-bonding network stabilizing the two long loops in the β-domain. It further accelerates the unfolding of this variant, starting from the hydrophobic core between the α- and β-domains. For the I56T variant, the introduction of a hydrophilic residue in the hydrophobic core directly destroys the native contacts in the α-β interface, leading to fast unfolding. The present results are consistent with the previous hypothesis suggesting that the distortion of the hydrophobic core at the α-β interface putatively results in the formation of the initial "seed" for amyloid fibrils.

Original languageEnglish
Pages (from-to)246-254
Number of pages9
JournalBiotechnology Progress
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 2007

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Familial Amyloidosis
amyloidosis
Muramidase
lysozyme
hydrogen bonding
molecular dynamics
structural proteins
Molecular Dynamics Simulation
Hydrogen Bonding
amyloid
Amyloid
Seeds
Ethanol
ethanol
Temperature
seeds
Population
temperature
Proteins

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Insights into the conformational changes of several human lysozyme variants associated with hereditary systemic amyloidosis. / Liu, Hsuan Liang; Wu, Yi Ching; Zhao, Jian Hua; Liu, Yi Fan; Huang, Chi Hung; Fang, Hsu Wei; Ho, Yih.

In: Biotechnology Progress, Vol. 23, No. 1, 01.2007, p. 246-254.

Research output: Contribution to journalArticle

Liu, Hsuan Liang ; Wu, Yi Ching ; Zhao, Jian Hua ; Liu, Yi Fan ; Huang, Chi Hung ; Fang, Hsu Wei ; Ho, Yih. / Insights into the conformational changes of several human lysozyme variants associated with hereditary systemic amyloidosis. In: Biotechnology Progress. 2007 ; Vol. 23, No. 1. pp. 246-254.
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