Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice

Frank C. Gibson, Charlie Hong, Hsin H. Chou, Hiromichi Yumoto, Jiqiu Chen, Egil Lien, Jodie Wong, Caroline Attardo Genco

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

Background - Infectious diseases have emerged as potential risk factors for cardiovascular disease (CVD). Epidemiological studies support a connection between periodontal disease, a chronic inflammatory disease of the supporting tissues of the teeth, and CVD. Methods and Results - To directly test the connection between periodontal disease and atherosclerosis, apoE-/- mice were orally challenged with the periodontal disease pathogen Porphyromonas gingivalis or an invasion-impaired P gingivalis fimbriae-deficient mutant (FimA-). Both wild-type P gingivalis and the FimA- mutant were detected in blood and aortic arch tissue of apoE-/- mice by PCR after challenge. ApoE-/- mice challenged with wild-type P gingivalis presented with increased atherosclerotic plaque and expressed the innate immune response markers Toll-like receptor (TLR)-2 and TLR-4 in aortic tissue. Despite detection of the FimA- mutant in the blood and in aortic arch tissue, apoE -/- mice challenged with the FimA- mutant did not present with periodontal disease, upregulation of TLRs, or accelerated atherosclerosis. Furthermore, we demonstrate that immunization to control P gingivalis-elicited periodontal disease concomitantly prevents P gingivalis-accelerated atherosclerosis. Conclusions - We conclude that invasive P gingivalis accelerates atherosclerosis.

Original languageEnglish
Pages (from-to)2801-2806
Number of pages6
JournalCirculation
Volume109
Issue number22
DOIs
Publication statusPublished - Jun 8 2004

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Periodontal Diseases
Apolipoproteins E
Atherosclerosis
Bacteria
Thoracic Aorta
Cardiovascular Diseases
Tooth Diseases
Toll-Like Receptor 2
Porphyromonas gingivalis
Toll-Like Receptor 4
Atherosclerotic Plaques
Innate Immunity
Communicable Diseases
Epidemiologic Studies
Immunization
Chronic Disease
Up-Regulation
Biomarkers
Polymerase Chain Reaction

Keywords

  • Atherosclerosis
  • Endothelium
  • Infection
  • Inflammation
  • Receptors

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. / Gibson, Frank C.; Hong, Charlie; Chou, Hsin H.; Yumoto, Hiromichi; Chen, Jiqiu; Lien, Egil; Wong, Jodie; Genco, Caroline Attardo.

In: Circulation, Vol. 109, No. 22, 08.06.2004, p. 2801-2806.

Research output: Contribution to journalArticle

Gibson, Frank C. ; Hong, Charlie ; Chou, Hsin H. ; Yumoto, Hiromichi ; Chen, Jiqiu ; Lien, Egil ; Wong, Jodie ; Genco, Caroline Attardo. / Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. In: Circulation. 2004 ; Vol. 109, No. 22. pp. 2801-2806.
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