Abstract
Background - Infectious diseases have emerged as potential risk factors for cardiovascular disease (CVD). Epidemiological studies support a connection between periodontal disease, a chronic inflammatory disease of the supporting tissues of the teeth, and CVD. Methods and Results - To directly test the connection between periodontal disease and atherosclerosis, apoE-/- mice were orally challenged with the periodontal disease pathogen Porphyromonas gingivalis or an invasion-impaired P gingivalis fimbriae-deficient mutant (FimA-). Both wild-type P gingivalis and the FimA- mutant were detected in blood and aortic arch tissue of apoE-/- mice by PCR after challenge. ApoE-/- mice challenged with wild-type P gingivalis presented with increased atherosclerotic plaque and expressed the innate immune response markers Toll-like receptor (TLR)-2 and TLR-4 in aortic tissue. Despite detection of the FimA- mutant in the blood and in aortic arch tissue, apoE -/- mice challenged with the FimA- mutant did not present with periodontal disease, upregulation of TLRs, or accelerated atherosclerosis. Furthermore, we demonstrate that immunization to control P gingivalis-elicited periodontal disease concomitantly prevents P gingivalis-accelerated atherosclerosis. Conclusions - We conclude that invasive P gingivalis accelerates atherosclerosis.
| Original language | English |
|---|---|
| Pages (from-to) | 2801-2806 |
| Number of pages | 6 |
| Journal | Circulation |
| Volume | 109 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - Jun 8 2004 |
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Keywords
- Atherosclerosis
- Endothelium
- Infection
- Inflammation
- Receptors
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. / Gibson, Frank C.; Hong, Charlie; Chou, Hsin H.; Yumoto, Hiromichi; Chen, Jiqiu; Lien, Egil; Wong, Jodie; Genco, Caroline Attardo.
In: Circulation, Vol. 109, No. 22, 08.06.2004, p. 2801-2806.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice
AU - Gibson, Frank C.
AU - Hong, Charlie
AU - Chou, Hsin H.
AU - Yumoto, Hiromichi
AU - Chen, Jiqiu
AU - Lien, Egil
AU - Wong, Jodie
AU - Genco, Caroline Attardo
PY - 2004/6/8
Y1 - 2004/6/8
N2 - Background - Infectious diseases have emerged as potential risk factors for cardiovascular disease (CVD). Epidemiological studies support a connection between periodontal disease, a chronic inflammatory disease of the supporting tissues of the teeth, and CVD. Methods and Results - To directly test the connection between periodontal disease and atherosclerosis, apoE-/- mice were orally challenged with the periodontal disease pathogen Porphyromonas gingivalis or an invasion-impaired P gingivalis fimbriae-deficient mutant (FimA-). Both wild-type P gingivalis and the FimA- mutant were detected in blood and aortic arch tissue of apoE-/- mice by PCR after challenge. ApoE-/- mice challenged with wild-type P gingivalis presented with increased atherosclerotic plaque and expressed the innate immune response markers Toll-like receptor (TLR)-2 and TLR-4 in aortic tissue. Despite detection of the FimA- mutant in the blood and in aortic arch tissue, apoE -/- mice challenged with the FimA- mutant did not present with periodontal disease, upregulation of TLRs, or accelerated atherosclerosis. Furthermore, we demonstrate that immunization to control P gingivalis-elicited periodontal disease concomitantly prevents P gingivalis-accelerated atherosclerosis. Conclusions - We conclude that invasive P gingivalis accelerates atherosclerosis.
AB - Background - Infectious diseases have emerged as potential risk factors for cardiovascular disease (CVD). Epidemiological studies support a connection between periodontal disease, a chronic inflammatory disease of the supporting tissues of the teeth, and CVD. Methods and Results - To directly test the connection between periodontal disease and atherosclerosis, apoE-/- mice were orally challenged with the periodontal disease pathogen Porphyromonas gingivalis or an invasion-impaired P gingivalis fimbriae-deficient mutant (FimA-). Both wild-type P gingivalis and the FimA- mutant were detected in blood and aortic arch tissue of apoE-/- mice by PCR after challenge. ApoE-/- mice challenged with wild-type P gingivalis presented with increased atherosclerotic plaque and expressed the innate immune response markers Toll-like receptor (TLR)-2 and TLR-4 in aortic tissue. Despite detection of the FimA- mutant in the blood and in aortic arch tissue, apoE -/- mice challenged with the FimA- mutant did not present with periodontal disease, upregulation of TLRs, or accelerated atherosclerosis. Furthermore, we demonstrate that immunization to control P gingivalis-elicited periodontal disease concomitantly prevents P gingivalis-accelerated atherosclerosis. Conclusions - We conclude that invasive P gingivalis accelerates atherosclerosis.
KW - Atherosclerosis
KW - Endothelium
KW - Infection
KW - Inflammation
KW - Receptors
UR - http://www.scopus.com/inward/record.url?scp=2942544238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942544238&partnerID=8YFLogxK
U2 - 10.1161/01.CIR.0000129769.17895.F0
DO - 10.1161/01.CIR.0000129769.17895.F0
M3 - Article
C2 - 15123526
AN - SCOPUS:2942544238
VL - 109
SP - 2801
EP - 2806
JO - Circulation
JF - Circulation
SN - 0009-7322
IS - 22
ER -