Inhibitory effects of wild bitter melon leaf extract on Propionibacterium acnes-induced skin inflammation in mice and cytokine production in vitro

Wen Cheng Huang, Tsung Hsien Tsai, Ching Jang Huang, You Yi Li, Jong Ho Chyuan, Lu Te Chuang, Po Jung Tsai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Propionibacterium acnes is a key pathogen involved in acne inflammation. Wild bitter melon (WBM, Momordica charantia L. var. abbreviate Seringe) is consumed as both a vegetable and as folk medicine in Taiwan. We examined the inhibitory activity of the total phenolic extract (TPE) of WBM leaf on P. acnes-induced inflammatory responses in vivo and in vitro. Our data showed that TPE significantly attenuated P. acnes-induced ear swelling in mice along with microabscess. Flow cytometry analysis revealed that TPE treatment significantly decreased the migration of neutrophils and interleukin (IL)-1β+ populations in vivo. In P. acnes-stimulated human monocytic THP-1 cells, TPE suppressed the mRNA levels and production of IL-8, IL-1β, and tumor necrosis factor (TNF)-α in vitro. In addition, TPE suppressed P. acnes-induced matrix metalloproteinase-9 levels. TPE blocked nuclear factor-κB (NF-κB) activation and inactivated mitogen-activated protein kinases (MAPK); these actions may partially account for its inhibitory effect on cytokine production. The quantitative HPLC analysis revealed gallic, chlorogenic, caffeic, ferulic, and cinnamic acids, myricetin, quercetin, luteolin, apigenin, and thymol in TPE. All these phenolics significantly suppressed P. acnes-induced IL-8 production in vitro. Our results suggest that WBM leaf extract effectively inhibits P. acnes-induced inflammatory responses and may be useful to relieve the inflammation of acne.

Original languageEnglish
Pages (from-to)2550-2560
Number of pages11
JournalFood and Function
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Momordica charantia
Propionibacterium acnes
skin (animal)
leaf extracts
cytokines
inflammation
Cytokines
Inflammation
Skin
mice
extracts
acne
ferulic acid
interleukin-8
Acne Vulgaris
interleukin-1
Interleukin-8
Interleukin-1
Thymol
Apigenin

ASJC Scopus subject areas

  • Food Science

Cite this

Inhibitory effects of wild bitter melon leaf extract on Propionibacterium acnes-induced skin inflammation in mice and cytokine production in vitro. / Huang, Wen Cheng; Tsai, Tsung Hsien; Huang, Ching Jang; Li, You Yi; Chyuan, Jong Ho; Chuang, Lu Te; Tsai, Po Jung.

In: Food and Function, Vol. 6, No. 8, 01.08.2015, p. 2550-2560.

Research output: Contribution to journalArticle

Huang, Wen Cheng ; Tsai, Tsung Hsien ; Huang, Ching Jang ; Li, You Yi ; Chyuan, Jong Ho ; Chuang, Lu Te ; Tsai, Po Jung. / Inhibitory effects of wild bitter melon leaf extract on Propionibacterium acnes-induced skin inflammation in mice and cytokine production in vitro. In: Food and Function. 2015 ; Vol. 6, No. 8. pp. 2550-2560.
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