Inhibitory effects of terpenoids from the fermented broth of the ascomycete stilbohypoxylon elaeicola YMJ173 on nitric oxide production in RAW264.7 macrophages

Guei Jane Wang, Wen Li Liang, Yu Ming Ju, Wen Bin Yang, Ya Wen Chang, Zong-Huei Li

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A series of six isopimarane-type diterpene glycosides, along with an eremophilane-type sesquiterpene, i.e., elaeicolasides A-C (1-3, resp.), 16-(α-D-mannopyranosyloxy)isopimar-7-en-19-oic acid (4), hymatoxin K (5), hymatoxin L (6), and elaeicolalactone (7), were isolated from the AcOEt extract of the fermented broth of Stilbohypoxylon elaeicola YMJ173. Among these, 1-3 and 7 are new compounds based on their spectroscopic data and sugar composition analysis. The effects of 1-7 on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated. All these compounds inhibited NO production, detected as nitrite in the culture medium, in activated macrophages without any cytotoxicity at a concentration of 100μM. Among these compounds, 2 showed a significant activity with the average maximum inhibition (E max) and median inhibitory concentration (IC 50) values of 93.3±0.5% and 79.3±0.4μM, respectively.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalChemistry and Biodiversity
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2012

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Keywords

  • Diterpenes
  • Eremophilane
  • Glycosides
  • Isopimarane
  • Sesquiterpenes
  • Stilbohypoxylon elaeicola

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Molecular Biology
  • Molecular Medicine

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