Inhibitory effects of plant growth regulators on xanthine oxidase

Shiow Yunn Sheu, Hsüch Ching Chiang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Several plant hormones and analogues were tested for their inhibitory effects on xanthine oxidase. The flavoprotein enzyme, xanthine oxidase, catalyses the oxidation of hypoxanthine to xanthine and then xanthine to uric acid which has λmax 295 nm. Uric acid was thus the basis for a spectrophotometric assay of the activity of xanthine oxidase. The results showed that trans-zeatin displayed the strongest activity (IC50= 23.5 μM) on xanthine oxidase inhibition, followed by indole-3-acrylic acid (IC50= 136.0 μM) and then by the mixed isomers of zeatin (trans-zeatin and cis-zeatin) (IC50= 198.65 μM). Trans-zeatin induced an uncompetitive inhibition of the enzyme with respect to the substrate xanthine and the apparent inhibition constant (Ki) was 5.09 μM. However, zeatin riboside was inactive. Since xanthine oxidase serum levels are increased in hepatitis, mild hepatic intoxication, tumours brain tissues, and DNA damage induced by cytotoxic agents, it is expected that trans-zeatin may be useful for the treatment of these diseases as well as gout which is caused by deposition of uric acid in the joints and oxidative damage of tissue caused by generation of superoxide union radical.

Original languageEnglish
Pages (from-to)311-316
Number of pages6
JournalAnticancer Research
Volume16
Issue number1
Publication statusPublished - Jan 1996

Fingerprint

Zeatin
Plant Growth Regulators
Xanthine Oxidase
Xanthine
Uric Acid
Inhibitory Concentration 50
Flavoproteins
Hypoxanthine
Gout
Cytotoxins
Enzymes
Brain Neoplasms
Superoxides
Hepatitis
DNA Damage
Joints
Liver
Serum

Keywords

  • Brain tumours
  • Cytokinins
  • DNA damage
  • Gout
  • Hepatitis
  • Plant growth regulator
  • Trans-zeatin
  • Xanthine oxidase inhibitor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Inhibitory effects of plant growth regulators on xanthine oxidase. / Sheu, Shiow Yunn; Chiang, Hsüch Ching.

In: Anticancer Research, Vol. 16, No. 1, 01.1996, p. 311-316.

Research output: Contribution to journalArticle

Sheu, Shiow Yunn ; Chiang, Hsüch Ching. / Inhibitory effects of plant growth regulators on xanthine oxidase. In: Anticancer Research. 1996 ; Vol. 16, No. 1. pp. 311-316.
@article{ee1a5236897247d0ac72ab90432d91f7,
title = "Inhibitory effects of plant growth regulators on xanthine oxidase",
abstract = "Several plant hormones and analogues were tested for their inhibitory effects on xanthine oxidase. The flavoprotein enzyme, xanthine oxidase, catalyses the oxidation of hypoxanthine to xanthine and then xanthine to uric acid which has λmax 295 nm. Uric acid was thus the basis for a spectrophotometric assay of the activity of xanthine oxidase. The results showed that trans-zeatin displayed the strongest activity (IC50= 23.5 μM) on xanthine oxidase inhibition, followed by indole-3-acrylic acid (IC50= 136.0 μM) and then by the mixed isomers of zeatin (trans-zeatin and cis-zeatin) (IC50= 198.65 μM). Trans-zeatin induced an uncompetitive inhibition of the enzyme with respect to the substrate xanthine and the apparent inhibition constant (Ki) was 5.09 μM. However, zeatin riboside was inactive. Since xanthine oxidase serum levels are increased in hepatitis, mild hepatic intoxication, tumours brain tissues, and DNA damage induced by cytotoxic agents, it is expected that trans-zeatin may be useful for the treatment of these diseases as well as gout which is caused by deposition of uric acid in the joints and oxidative damage of tissue caused by generation of superoxide union radical.",
keywords = "Brain tumours, Cytokinins, DNA damage, Gout, Hepatitis, Plant growth regulator, Trans-zeatin, Xanthine oxidase inhibitor",
author = "Sheu, {Shiow Yunn} and Chiang, {Hs{\"u}ch Ching}",
year = "1996",
month = "1",
language = "English",
volume = "16",
pages = "311--316",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "1",

}

TY - JOUR

T1 - Inhibitory effects of plant growth regulators on xanthine oxidase

AU - Sheu, Shiow Yunn

AU - Chiang, Hsüch Ching

PY - 1996/1

Y1 - 1996/1

N2 - Several plant hormones and analogues were tested for their inhibitory effects on xanthine oxidase. The flavoprotein enzyme, xanthine oxidase, catalyses the oxidation of hypoxanthine to xanthine and then xanthine to uric acid which has λmax 295 nm. Uric acid was thus the basis for a spectrophotometric assay of the activity of xanthine oxidase. The results showed that trans-zeatin displayed the strongest activity (IC50= 23.5 μM) on xanthine oxidase inhibition, followed by indole-3-acrylic acid (IC50= 136.0 μM) and then by the mixed isomers of zeatin (trans-zeatin and cis-zeatin) (IC50= 198.65 μM). Trans-zeatin induced an uncompetitive inhibition of the enzyme with respect to the substrate xanthine and the apparent inhibition constant (Ki) was 5.09 μM. However, zeatin riboside was inactive. Since xanthine oxidase serum levels are increased in hepatitis, mild hepatic intoxication, tumours brain tissues, and DNA damage induced by cytotoxic agents, it is expected that trans-zeatin may be useful for the treatment of these diseases as well as gout which is caused by deposition of uric acid in the joints and oxidative damage of tissue caused by generation of superoxide union radical.

AB - Several plant hormones and analogues were tested for their inhibitory effects on xanthine oxidase. The flavoprotein enzyme, xanthine oxidase, catalyses the oxidation of hypoxanthine to xanthine and then xanthine to uric acid which has λmax 295 nm. Uric acid was thus the basis for a spectrophotometric assay of the activity of xanthine oxidase. The results showed that trans-zeatin displayed the strongest activity (IC50= 23.5 μM) on xanthine oxidase inhibition, followed by indole-3-acrylic acid (IC50= 136.0 μM) and then by the mixed isomers of zeatin (trans-zeatin and cis-zeatin) (IC50= 198.65 μM). Trans-zeatin induced an uncompetitive inhibition of the enzyme with respect to the substrate xanthine and the apparent inhibition constant (Ki) was 5.09 μM. However, zeatin riboside was inactive. Since xanthine oxidase serum levels are increased in hepatitis, mild hepatic intoxication, tumours brain tissues, and DNA damage induced by cytotoxic agents, it is expected that trans-zeatin may be useful for the treatment of these diseases as well as gout which is caused by deposition of uric acid in the joints and oxidative damage of tissue caused by generation of superoxide union radical.

KW - Brain tumours

KW - Cytokinins

KW - DNA damage

KW - Gout

KW - Hepatitis

KW - Plant growth regulator

KW - Trans-zeatin

KW - Xanthine oxidase inhibitor

UR - http://www.scopus.com/inward/record.url?scp=0029960884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029960884&partnerID=8YFLogxK

M3 - Article

C2 - 8615627

AN - SCOPUS:0029960884

VL - 16

SP - 311

EP - 316

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 1

ER -