Inhibitory effects of Phyllanthus emblica L. on hepatic steatosis and liver fibrosis in vitro

Chi Cheng Lu, Shu Han Yang, Shih Min Hsia, Chi Hao Wu, Gow Chin Yen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Phyllanthus emblica L. exerts multiple biological functions, and its fruit possesses hepatoprotective benefits. However, the underlying mechanisms of these activities remain unclear. This study aimed to investigate the potential impact of P. emblica on improving nonalcoholic fatty liver disease (NAFLD). Free fatty acid mixture-induced HepG2 cells and leptin-induced HSC-T6 hepatic stellate cells were used to mimic nonalcoholic steatohepatitis and hepatic fibrosis, respectively. Ellagic acid (EA) was identified as a major compound in the water extract of P. emblica fruits (WEPL). WEPL significantly decreased fat accumulation and ROS production by altering lipogenesis-related gene expression and stimulating AMP-activated protein kinase (AMPK) signalling in treated HepG2 cells. WEPL also inhibited hepatic fibrosis in HSC-T6 cells and induced mitochondrial apoptosis. Importantly, EA was shown to inhibit cellular steatosis and liver fibrosis in vitro. In conclusion, WEPL reduces lipogenesis in hepatocytes and fibrotic cells, suggesting that WEPL has the potential to improve NAFLD.

Original languageEnglish
Pages (from-to)20-30
Number of pages11
JournalJournal of Functional Foods
Volume20
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Phyllanthus emblica
liver cirrhosis
fatty liver
Fatty Liver
Liver Cirrhosis
Ellagic Acid
Lipogenesis
Hep G2 Cells
Liver
Fruit
Fibrosis
ellagic acid
lipogenesis
Hepatic Stellate Cells
AMP-Activated Protein Kinases
cells
Leptin
Nonesterified Fatty Acids
Hepatocytes
AMP-activated protein kinase

Keywords

  • Free fatty acid
  • Hepatic fibrosis
  • Leptin
  • NAFLD
  • Phyllanthus emblica

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Inhibitory effects of Phyllanthus emblica L. on hepatic steatosis and liver fibrosis in vitro. / Lu, Chi Cheng; Yang, Shu Han; Hsia, Shih Min; Wu, Chi Hao; Yen, Gow Chin.

In: Journal of Functional Foods, Vol. 20, 01.01.2016, p. 20-30.

Research output: Contribution to journalArticle

Lu, Chi Cheng ; Yang, Shu Han ; Hsia, Shih Min ; Wu, Chi Hao ; Yen, Gow Chin. / Inhibitory effects of Phyllanthus emblica L. on hepatic steatosis and liver fibrosis in vitro. In: Journal of Functional Foods. 2016 ; Vol. 20. pp. 20-30.
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