Inhibitory Effects of Momordicine I on High-Glucose-Induced Cell Proliferation and Collagen Synthesis in Rat Cardiac Fibroblasts

Po Yuan Chen, Neng Lang Shih, Wen Rui Hao, Chun Chao Chen, Ju Chi Liu, Li Chin Sung

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Diabetes-associated cardiac fibrosis is a severe cardiovascular complication. Momordicine I, a bioactive triterpenoid isolated from bitter melon, has been demonstrated to have antidiabetic properties. This study investigated the effects of momordicine I on high-glucose-induced cardiac fibroblast activation. Rat cardiac fibroblasts were cultured in a high-glucose (25 mM) medium in the absence or presence of momordicine I, and the changes in collagen synthesis, transforming growth factor-β1 (TGF-β1) production, and related signaling molecules were assessed. Increased oxidative stress plays a critical role in the development of high-glucose-induced cardiac fibrosis; we further explored momordicine I's antioxidant activity and its effect on fibroblasts. Our data revealed that a high-glucose condition promoted fibroblast proliferation and collagen synthesis and these effects were abolished by momordicine I (0.3 and 1 μM) pretreatment. Furthermore, the inhibitory effect of momordicine I on high-glucose-induced fibroblast activation may be associated with its activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the inhibition of reactive oxygen species formation, TGF-β1 production, and Smad2/3 phosphorylation. The addition of brusatol (a selective inhibitor of Nrf2) or Nrf2 siRNA significantly abolished the inhibitory effect of momordicine I on fibroblast activation. Our findings revealed that the antifibrotic effect of momordicine I was mediated, at least partially, by the inhibition of the TGF-β1/Smad pathway, fibroblast proliferation, and collagen synthesis through Nrf2 activation. Thus, this work provides crucial insights into the molecular pathways for the clinical application of momordicine I for treating diabetes-associated cardiac fibrosis.

Original languageEnglish
Number of pages1
JournalOxidative Medicine and Cellular Longevity
Volume2018
DOIs
Publication statusPublished - Jan 1 2018

Keywords

  • Animals
  • Antioxidants/pharmacology
  • Cell Proliferation/drug effects
  • Collagen/biosynthesis
  • Fibroblasts/drug effects
  • Glucose/toxicity
  • Models, Biological
  • Myocardium/pathology
  • NF-E2-Related Factor 2/metabolism
  • Phosphorylation/drug effects
  • Quassins/pharmacology
  • RNA, Small Interfering/metabolism
  • Rats, Sprague-Dawley
  • Reactive Oxygen Species/metabolism
  • Signal Transduction/drug effects
  • Smad Proteins/metabolism
  • Sterols/chemistry
  • Transforming Growth Factor beta1/metabolism

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Cell Biology

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