Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells

Hsu Wei Fang, Shiuh Bin Fang, Jen Shiu Chiang Chiau, Chun Yan Yeung, Wai Tao Chan, Chuen Bin Jiang, Mei Lien Cheng, Hung Chang Lee

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40 Citations (Scopus)


In this study, we investigated the anti-inflammatory and reinforcing barrier effects of Lactobacillus casei subsp. rhamnosus (Lcr35) on Caco-2 intestinal epithelial cells already exposed to Salmonella LPS. Using the Transwell co-culture model, Salmonella LPS was apically added to polarized Caco-2 cells co-cultured with peripheral blood mononuclear cells (PBMCs) in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with Lcr35 for 1, 6, 24 or 48 h. Apical inoculation of Lcr35 after 48 h significantly inhibited the basolateral secretion of interleukin-8 (IL-8) in the Caco-2/PBMC co-culture. The PCR analysis showed that Lcr35 significantly downregulated mRNA expression of monocyte chemoattractant protein 1 (MCP-1) (P

Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalJournal of Medical Microbiology
Issue number5
Publication statusPublished - May 2010
Externally publishedYes


ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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