Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells

Hsu Wei Fang, Shiuh Bin Fang, Jen Shiu Chiang Chiau, Chun Yan Yeung, Wai Tao Chan, Chuen Bin Jiang, Mei Lien Cheng, Hung Chang Lee

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In this study, we investigated the anti-inflammatory and reinforcing barrier effects of Lactobacillus casei subsp. rhamnosus (Lcr35) on Caco-2 intestinal epithelial cells already exposed to Salmonella LPS. Using the Transwell co-culture model, Salmonella LPS was apically added to polarized Caco-2 cells co-cultured with peripheral blood mononuclear cells (PBMCs) in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with Lcr35 for 1, 6, 24 or 48 h. Apical inoculation of Lcr35 after 48 h significantly inhibited the basolateral secretion of interleukin-8 (IL-8) in the Caco-2/PBMC co-culture. The PCR analysis showed that Lcr35 significantly downregulated mRNA expression of monocyte chemoattractant protein 1 (MCP-1) (P

Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalJournal of Medical Microbiology
Volume59
Issue number5
DOIs
Publication statusPublished - May 2010
Externally publishedYes

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Lactobacillus casei
Caco-2 Cells
Coculture Techniques
Salmonella
Lipopolysaccharides
Blood Cells
Inflammation
Chemokine CCL2
Interleukin-8
Anti-Inflammatory Agents
Down-Regulation
Cell Culture Techniques
Epithelial Cells
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells. / Fang, Hsu Wei; Fang, Shiuh Bin; Chiau, Jen Shiu Chiang; Yeung, Chun Yan; Chan, Wai Tao; Jiang, Chuen Bin; Cheng, Mei Lien; Lee, Hung Chang.

In: Journal of Medical Microbiology, Vol. 59, No. 5, 05.2010, p. 573-579.

Research output: Contribution to journalArticle

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AU - Cheng, Mei Lien

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