Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells

Hsu Wei Fang; Shiuh Bin Fang; Jen Shiu Chiang Chiau; Chun Yan Yeung; Wai Tao Chan; Chuen Bin Jiang; Mei Lien Cheng; Hung Chang Lee

doi:10.1099/jmm.0.009662-0

Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells

Hsu Wei Fang, Shiuh Bin Fang, Jen Shiu Chiang Chiau, Chun Yan Yeung, Wai Tao Chan, Chuen Bin Jiang, Mei Lien Cheng, Hung Chang Lee

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

In this study, we investigated the anti-inflammatory and reinforcing barrier effects of Lactobacillus casei subsp. rhamnosus (Lcr35) on Caco-2 intestinal epithelial cells already exposed to Salmonella LPS. Using the Transwell co-culture model, Salmonella LPS was apically added to polarized Caco-2 cells co-cultured with peripheral blood mononuclear cells (PBMCs) in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with Lcr35 for 1, 6, 24 or 48 h. Apical inoculation of Lcr35 after 48 h significantly inhibited the basolateral secretion of interleukin-8 (IL-8) in the Caco-2/PBMC co-culture. The PCR analysis showed that Lcr35 significantly downregulated mRNA expression of monocyte chemoattractant protein 1 (MCP-1) (P

Original language	English
Pages (from-to)	573-579
Number of pages	7
Journal	Journal of Medical Microbiology
Volume	59
Issue number	5
DOIs	https://doi.org/10.1099/jmm.0.009662-0
Publication status	Published - May 2010
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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Fang, H. W., Fang, S. B., Chiau, J. S. C., Yeung, C. Y., Chan, W. T., Jiang, C. B., Cheng, M. L., & Lee, H. C. (2010). Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells. Journal of Medical Microbiology, 59(5), 573-579. https://doi.org/10.1099/jmm.0.009662-0

Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells. / Fang, Hsu Wei; Fang, Shiuh Bin; Chiau, Jen Shiu Chiang et al.

In: Journal of Medical Microbiology, Vol. 59, No. 5, 05.2010, p. 573-579.

Research output: Contribution to journal › Article › peer-review

Fang, HW, Fang, SB, Chiau, JSC, Yeung, CY, Chan, WT, Jiang, CB, Cheng, ML & Lee, HC 2010, 'Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells', Journal of Medical Microbiology, vol. 59, no. 5, pp. 573-579. https://doi.org/10.1099/jmm.0.009662-0

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abstract = "In this study, we investigated the anti-inflammatory and reinforcing barrier effects of Lactobacillus casei subsp. rhamnosus (Lcr35) on Caco-2 intestinal epithelial cells already exposed to Salmonella LPS. Using the Transwell co-culture model, Salmonella LPS was apically added to polarized Caco-2 cells co-cultured with peripheral blood mononuclear cells (PBMCs) in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with Lcr35 for 1, 6, 24 or 48 h. Apical inoculation of Lcr35 after 48 h significantly inhibited the basolateral secretion of interleukin-8 (IL-8) in the Caco-2/PBMC co-culture. The PCR analysis showed that Lcr35 significantly downregulated mRNA expression of monocyte chemoattractant protein 1 (MCP-1) (P",

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Inhibitory effects of Lactobacillus casei subsp. rhamnosus on Salmonella lipopolysaccharide-induced inflammation and epithelial barrier dysfunction in a co-culture model using Caco-2/peripheral blood mononuclear cells

Abstract

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