Inhibitory effects of glycosides from the leaves of Melaleuca quinquenervia on vascular contraction of rats

Tzong Huei Lee, Guei Jane Wang, Ching Kuo Lee, Yueh Hsiung Kuo, Chang Hung Chou

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Two new glycosides, 3-hydroxy-5-methoxy-4-methylphenyl β-D-glucopyranoside (1) and 4-benzoyl-2-C-β-glucopyranosyl-3,5-dihydroxy-6-methylphenyl β-D-glucopyranoside (2), together with four known glycosides, 2-endo-β-o-glucopyranosyloxy-1,8-cineole (3a), 2-exo-β-D-glucopyranosyloxy-1,8-cineole (3b), roseoside (4), and citroside A (5), were isolated from the methanolic extract of leaves of Melaleuca quinquenervia. Their structures were elucidated on the basis of spectroscopic analysis. Compounds 1, 2a and 3 inhibited contractile response induced by phenylephrine in aortic rings from Sprague-Dawley rats. This inhibition was independent of the endothelium. Compounds 2 and 4 significantly relaxed precontracted aortic rings, in an endothelium-dependent manner. Pretreatment of Nω-nitro-L-arginine (L-NNA), a nitric oxide synthase inhibitor, partially attenuated the vasorelaxation induced by both compounds, suggesting that nitric oxide was likely the responsible mediator. The rank-order potency (EC50 value) of vasorelaxing activities of these compounds is 4 > 2 > 2a > 3 > 1.

Original languageEnglish
Pages (from-to)492-496
Number of pages5
JournalPlanta Medica
Issue number6
Publication statusPublished - 2002
Externally publishedYes


  • Glycosides
  • Leaves
  • Melaleuca quinquenervia
  • Myrtaceae
  • Vaso-relaxing activities

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology


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