Inhibitory effects of glycosides from the leaves of Melaleuca quinquenervia on vascular contraction of rats

Tzong Huei Lee, Guei Jane Wang, Ching Kuo Lee, Yueh Hsiung Kuo, Chang Hung Chou

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two new glycosides, 3-hydroxy-5-methoxy-4-methylphenyl β-D-glucopyranoside (1) and 4-benzoyl-2-C-β-glucopyranosyl-3,5-dihydroxy-6-methylphenyl β-D-glucopyranoside (2), together with four known glycosides, 2-endo-β-o-glucopyranosyloxy-1,8-cineole (3a), 2-exo-β-D-glucopyranosyloxy-1,8-cineole (3b), roseoside (4), and citroside A (5), were isolated from the methanolic extract of leaves of Melaleuca quinquenervia. Their structures were elucidated on the basis of spectroscopic analysis. Compounds 1, 2a and 3 inhibited contractile response induced by phenylephrine in aortic rings from Sprague-Dawley rats. This inhibition was independent of the endothelium. Compounds 2 and 4 significantly relaxed precontracted aortic rings, in an endothelium-dependent manner. Pretreatment of Nω-nitro-L-arginine (L-NNA), a nitric oxide synthase inhibitor, partially attenuated the vasorelaxation induced by both compounds, suggesting that nitric oxide was likely the responsible mediator. The rank-order potency (EC50 value) of vasorelaxing activities of these compounds is 4 > 2 > 2a > 3 > 1.

Original languageEnglish
Pages (from-to)492-496
Number of pages5
JournalPlanta Medica
Volume68
Issue number6
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Melaleuca
Melaleuca quinquenervia
cineole
Glycosides
endothelium
blood vessels
Endothelium
Blood Vessels
glycosides
Rats
phenylephrine
Spectroscopic analysis
vasodilation
rats
Phenylephrine
nitric oxide synthase
Vasodilation
Nitric Oxide Synthase
leaf extracts
nitric oxide

Keywords

  • Glycosides
  • Leaves
  • Melaleuca quinquenervia
  • Myrtaceae
  • Vaso-relaxing activities

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Inhibitory effects of glycosides from the leaves of Melaleuca quinquenervia on vascular contraction of rats. / Lee, Tzong Huei; Wang, Guei Jane; Lee, Ching Kuo; Kuo, Yueh Hsiung; Chou, Chang Hung.

In: Planta Medica, Vol. 68, No. 6, 2002, p. 492-496.

Research output: Contribution to journalArticle

Lee, Tzong Huei ; Wang, Guei Jane ; Lee, Ching Kuo ; Kuo, Yueh Hsiung ; Chou, Chang Hung. / Inhibitory effects of glycosides from the leaves of Melaleuca quinquenervia on vascular contraction of rats. In: Planta Medica. 2002 ; Vol. 68, No. 6. pp. 492-496.
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