Inhibitory effects of Cassia tora L. on benzo[a]pyrene-mediated DNA damage toward HepG2 cells

Chi Hao Wu, Chiu Lan Hsieh, Tuzz Ying Song, Gow Chin Yen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effects of water extracts from Cassia tora L. (WECT) treated with different degrees of roasting on benzo[a]pyrene (B[a]P)-induced DNA damage in human hepatoma cell line HepG2 were investigated via the comet assay without exogenous activation mixtures, such as S9 mix. WECT alone, at concentrations of 0.1-2 mg/mL, showed neither cytotoxic nor genotoxic effect toward HepG2 cells. B[a]P-induced DNA damage in HepG2 cells could be reduced by WECT in a dose-dependent manner (P <0.05). At a concentration of 1 mg/mL, the inhibitory effects of WECT on DNA damage were in the order unroasted (72%) > roasted at 150 °C (60%) > roasted at 250 °C (23%). Ethoxyresorufin-O-dealkylase activity of HepG2 cells was effectively inhibited by WECT, and a similar trend of inhibition was observed in the order unroasted (64%) > roasted at 150 °C (42%) > roasted at 250 °C (18%). The activity of NADPH cytochrome P-450 reductase was also decreased by unroasted and 150 °C-roasted samples (50% and 38%, respectively). Furthermore, glutathione S-transferase activity was increased by treatment with unroasted (1.26-fold) and 150 °C-roasted (1.35-fold) samples at 1 mg/mL. In addition, the contents of anthraquinones (AQs) in WECT, including chrysophanol, emodin, and rhein, were decreased with increasing roasting temperature. Each of these AQs also demonstrated significant antigenotoxic activity in the comet assay. The inhibitory effects of chrysophanol, emodin, and rhein on B[a]P-mediated DNA damage in HepG2 cells were 78, 86, and 71%, respectively, at 100 μM. These findings suggested that the decreased antigenotoxicity of the roasted samples might be due to a reduction in their AQs content.

Original languageEnglish
Pages (from-to)2579-2586
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume49
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Senna tora
Cassia
Benzo(a)pyrene
Hep G2 Cells
DNA damage
DNA Damage
Anthraquinones
anthraquinones
Water
Emodin
DNA
extracts
emodin
Comet Assay
roasting
water
cells
Assays
NADPH-Ferrihemoprotein Reductase
Cytochrome P-450 CYP1A1

Keywords

  • Anthraquinones
  • Antigenotoxicity
  • Benzo[a]pyrene
  • Cassia tora L.
  • Comet assay
  • DNA damage
  • HepG2

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Inhibitory effects of Cassia tora L. on benzo[a]pyrene-mediated DNA damage toward HepG2 cells. / Wu, Chi Hao; Hsieh, Chiu Lan; Song, Tuzz Ying; Yen, Gow Chin.

In: Journal of Agricultural and Food Chemistry, Vol. 49, No. 5, 2001, p. 2579-2586.

Research output: Contribution to journalArticle

Wu, Chi Hao ; Hsieh, Chiu Lan ; Song, Tuzz Ying ; Yen, Gow Chin. / Inhibitory effects of Cassia tora L. on benzo[a]pyrene-mediated DNA damage toward HepG2 cells. In: Journal of Agricultural and Food Chemistry. 2001 ; Vol. 49, No. 5. pp. 2579-2586.
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abstract = "The effects of water extracts from Cassia tora L. (WECT) treated with different degrees of roasting on benzo[a]pyrene (B[a]P)-induced DNA damage in human hepatoma cell line HepG2 were investigated via the comet assay without exogenous activation mixtures, such as S9 mix. WECT alone, at concentrations of 0.1-2 mg/mL, showed neither cytotoxic nor genotoxic effect toward HepG2 cells. B[a]P-induced DNA damage in HepG2 cells could be reduced by WECT in a dose-dependent manner (P <0.05). At a concentration of 1 mg/mL, the inhibitory effects of WECT on DNA damage were in the order unroasted (72{\%}) > roasted at 150 °C (60{\%}) > roasted at 250 °C (23{\%}). Ethoxyresorufin-O-dealkylase activity of HepG2 cells was effectively inhibited by WECT, and a similar trend of inhibition was observed in the order unroasted (64{\%}) > roasted at 150 °C (42{\%}) > roasted at 250 °C (18{\%}). The activity of NADPH cytochrome P-450 reductase was also decreased by unroasted and 150 °C-roasted samples (50{\%} and 38{\%}, respectively). Furthermore, glutathione S-transferase activity was increased by treatment with unroasted (1.26-fold) and 150 °C-roasted (1.35-fold) samples at 1 mg/mL. In addition, the contents of anthraquinones (AQs) in WECT, including chrysophanol, emodin, and rhein, were decreased with increasing roasting temperature. Each of these AQs also demonstrated significant antigenotoxic activity in the comet assay. The inhibitory effects of chrysophanol, emodin, and rhein on B[a]P-mediated DNA damage in HepG2 cells were 78, 86, and 71{\%}, respectively, at 100 μM. These findings suggested that the decreased antigenotoxicity of the roasted samples might be due to a reduction in their AQs content.",
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