Abstract

The myocardial protective effects of trilinolein, isolated from the Chinese herb Sanchi (Panax notoginseng), may be related to its antioxidant effects. In the present study, we investigated the effects of trilinolein on angiotensin II-induced cardiomyocyte hypertrophy. Cultured neonatal rat cardiomyocytes were stimulated with angiotensin II, [3H]leucine incorporation and the β-myosin heavy chain promoter activity were examined. We also examined the effects of trilinolein on angiotensin II-induced intracellular reactive oxygen species generation. Trilinolein significantly inhibited angiotensin II-increased protein synthesis, β-myosin heavy chain promoter activity, and intracellular reactive oxygen species generation. Antioxidant N-acetylcysteine also decreased angiotensin II-increased protein synthesis and β-myosin heavy chain promoter activity. Furthermore, trilinolein and N-acetylcysteine decreased angiotensin II- or hydrogen peroxide (H2O2)-activated mitogen-activated protein kinases (MAPKs) phosphorylation, and activator protein-1 (AP-1)- [or nuclear factor-κB (NF-κB)]-reporter activities. These data indicate that trilinolein inhibits angiotensin II-induced cardiomyocyte hypertrophy and β-myosin heavy chain promoter activity via attenuation of reactive oxygen species generation.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalEuropean Journal of Pharmacology
Volume484
Issue number1
DOIs
Publication statusPublished - Jan 19 2004

Fingerprint

Cardiac Myocytes
Angiotensin II
Hypertrophy
Myosin Heavy Chains
Reactive Oxygen Species
Acetylcysteine
Antioxidants
Panax notoginseng
Transcription Factor AP-1
trilinolein
Mitogen-Activated Protein Kinases
Leucine
Hydrogen Peroxide
Proteins
Phosphorylation

Keywords

  • β-myosin heavy chain
  • Angiotensin II
  • Cardiomyocyte hypertrophy
  • MAP (mitogen-activated protein) kinase
  • Reactive oxygen species
  • Trilinolein

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Inhibitory effect of trilinolein on angiotensin II-induced cardiomyocyte hypertrophy. / Liu, Ju Chi; Cheng, Tzu Hurng; Lee, Horng Mo; Lee, Wen Sen; Shih, Neng Lang; Chen, Yen Ling; Chen, Jin Jer; Chan, Paul.

In: European Journal of Pharmacology, Vol. 484, No. 1, 19.01.2004, p. 1-8.

Research output: Contribution to journalArticle

Liu, Ju Chi ; Cheng, Tzu Hurng ; Lee, Horng Mo ; Lee, Wen Sen ; Shih, Neng Lang ; Chen, Yen Ling ; Chen, Jin Jer ; Chan, Paul. / Inhibitory effect of trilinolein on angiotensin II-induced cardiomyocyte hypertrophy. In: European Journal of Pharmacology. 2004 ; Vol. 484, No. 1. pp. 1-8.
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