Inhibitory effect of resveratrol on angiotensin II-induced cardiomyocyte hypertrophy

Tzu Hurng Cheng, Ju Chi Liu, Heng Lin, Neng Lang Shih, Yen Ling Chen, Meng Ting Huang, Paul Chan, Ching Feng Cheng, Jin Jer Chen

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Resveratrol is proposed to account in part for the protective effect of red wine on the cardiovascular system. Angiotensin II (Ang II) is a potent hypertrophic stimulus in cardiomyocytes. In this study, we determined the effect of resveratrol on Ang II-induced cardiomyocyte hypertrophy. Cultured neonatal rat cardiomyocytes were stimulated with Ang II, and [ 3H]leucine incorporation and β-myosin heavy chain (β-MyHC) promoter activity were examined. Intracellular reactive oxygen species (ROS) were measured by a redox-sensitive fluorescent dye, 2′ 7′-dichlorofluorescin diacetate, and the extracellular signal-regulated kinase (ERK) phosphorylation was examined by Western blotting. Resveratrol inhibited Ang II-increased intracellular ROS levels. Furthermore, resveratrol, as well as the antioxidant N-acetyl-cysteine, decreased Ang II- or H 2O 2-increased protein synthesis, β-MyHC promoter activity, and ERK phosphorylation. In summary, we demonstrate for the first time that resveratrol inhibits Ang II-induced cardiomyocyte hypertrophy via attenuation of ROS generation.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume369
Issue number2
DOIs
Publication statusPublished - Feb 2004

Keywords

  • β-Myosin heavy chain
  • Angiotensin II
  • Cardiomyocyte hypertrophy
  • Extracellular signal-regulated kinase
  • Reactive oxygen species
  • Resveratrol

ASJC Scopus subject areas

  • Pharmacology

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