Inhibitory activities of semicarbazide-sensitive amine oxidase and angiotensin converting enzyme of pectin hydroxamic acid

Wen Chi Hou, Mei Hsien Lee, Feng-Lin Hsu, Yaw Huei Lin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Solutions of 100 mL of 1% commercial pectin each with a different degree of esterification (DE), DE94, DE65, and DE25, were reacted with 100 mL of 2 M alkaline hydroxylamine (pH 12.0) at room temperature for 4 or 18 h. These pectin hydroxamic acids (PHAs; DE94T4, DE94T18, DE65T4, and DE25T4) were used to test the inhibitory activities against semicarbazide-sensitive amine oxidase (SSAO) and angiotensin-converting enzyme (ACE). Compared to different DE pectins (DE94, DE65, and DE25), the PHAs of DE94T4, DE94T18, DE65T4, and DE25T4 showed different inhibition activities against SSAO or ACE. Commercial pectins with different DE values showed negligible SSAO or ACE inhibitions. The order of SSAO inhibition was DE65T4 > DE94T18 ≈ DE25T4 ≫ DE94T4. However, the order of ACE inhibition was DE94T4 > DE94T18 ≫ DE65T4 > DE25T4. The SSAO activity staining or ACE-hydrolyzed products on TLC chromatogram also confirmed the inhibitory activities of PHAs against SSAO or ACE.

Original languageEnglish
Pages (from-to)6362-6366
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number21
DOIs
Publication statusPublished - Oct 8 2003

Fingerprint

hydroxamic acids
Hydroxamic Acids
Amine Oxidase (Copper-Containing)
peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
amines
pectins
Enzyme inhibition
Esterification
esterification
Pectins
enzyme inhibition
Hydroxylamine
pectin
ambient temperature
Staining and Labeling
Temperature

Keywords

  • Angiotensin converting enzyme (ACE)
  • Degree of esterification (DE)
  • Pectin
  • Pectin hydroxamic acid (PHA)
  • Semicarbazide-sensitive amine oxidase (SSAO)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Inhibitory activities of semicarbazide-sensitive amine oxidase and angiotensin converting enzyme of pectin hydroxamic acid. / Hou, Wen Chi; Lee, Mei Hsien; Hsu, Feng-Lin; Lin, Yaw Huei.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 21, 08.10.2003, p. 6362-6366.

Research output: Contribution to journalArticle

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