Inhibition of xanthine oxidase by cytokinins and related substances

Shiow Yunn Sheu, Y. U Chan Lin, Hsüch Ching Chiang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Fourteen cytokinins were tested for their inhibitory effects on xanthine oxidase. The enzyme, xanthine oxidase catalyses the oxidation of hypoxanthine to xanthine and of xanthine to uric acid which has λ(max) of 295 nm, forming the basis for a spectrophotometric assay of the activity of xanthine oxidase. The results showed that adenine-HCl, N 6-(2-isopentenyl)-adenine, purine and DL-dihydrozeatin displayed very potent activities (IC 50 = 1.92, 10.99, 60.98 and 86.36 μM respectively). Their apparent inhibition constants (Ki) were 2.20, 17.99, 13.59 and 115.62 μM, and induced competitive, uncompetitive, competitive and non-competitive type inhibitors respectively with respect to the substrate xanthine.

Original languageEnglish
Pages (from-to)3571-3576
Number of pages6
JournalAnticancer Research
Volume16
Issue number6 B
Publication statusPublished - 1996

Fingerprint

Cytokinins
Xanthine
Xanthine Oxidase
Adenine
Hypoxanthine
Uric Acid
Enzymes

Keywords

  • Adenine HCl
  • Cytokinins
  • Dihydrozeatin
  • DNA damage
  • Gout
  • Hepatitis
  • Leukemia
  • N -(2-isopentenyl)-adenine
  • Purine
  • Tumours
  • Xanthine oxidase inhibitor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sheu, S. Y., Lin, Y. U. C., & Chiang, H. C. (1996). Inhibition of xanthine oxidase by cytokinins and related substances. Anticancer Research, 16(6 B), 3571-3576.

Inhibition of xanthine oxidase by cytokinins and related substances. / Sheu, Shiow Yunn; Lin, Y. U Chan; Chiang, Hsüch Ching.

In: Anticancer Research, Vol. 16, No. 6 B, 1996, p. 3571-3576.

Research output: Contribution to journalArticle

Sheu, SY, Lin, YUC & Chiang, HC 1996, 'Inhibition of xanthine oxidase by cytokinins and related substances', Anticancer Research, vol. 16, no. 6 B, pp. 3571-3576.
Sheu, Shiow Yunn ; Lin, Y. U Chan ; Chiang, Hsüch Ching. / Inhibition of xanthine oxidase by cytokinins and related substances. In: Anticancer Research. 1996 ; Vol. 16, No. 6 B. pp. 3571-3576.
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