Inhibition of xanthine oxidase by acacia confusa extracts and their phytochemicals

Yu Tang Tung, Shang Tzen Chang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Acacia confusa Merr. (Leguminosae) is traditionally used as a medicinal plant in Taiwan. In the present study, the XOD-inhibitory activity of ethanolic extracts from A. confusa was investigated for the first time. Results demonstrated that the ethanolic extract of A. confusa heartwood had a strong XOD-inhibitory activity. Among all fractions derived from heartwood extracts, the EtOAc fraction exhibited the best inhibitory activity. Following column chromatography and reverse-phase highperformance liquid chromatography, eight specific phytochemicals including melanoxetin, 7,8,3′, 4′-tetrahydroxyflavone, transilitin, okanin, 3,7,8,3′-tetrahydroxy- 4'-methoxyflavone, 7,8,3′-trihydroxy3,4'-dimethoxyflavone, 7,3′,4′-trihydroxyflavone, and 7,3′,4′-trihydroxy-3- methoxyflavone were isolated and identified from the EtOAc fraction. In addition, the IC 50 values indicated that okanin showed the strongest XOD-inhibitory effect (IC 50 value of 0.076 μM), followed by melanoxetin (0.274 μM) and allopurinol (4.784 μM). The present study revealed that okanin and melanoxetin showed excellent inhibition on XOD in noncompetitive and competitive mode, respectively, and their inhibitory activity is better than that of allopurinol. This is the first study that demonstrates the XOD-inhibitory performance of okanin and melanoxetin.

Original languageEnglish
Pages (from-to)781-786
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number2
DOIs
Publication statusPublished - Jan 27 2010
Externally publishedYes

Fingerprint

Acacia confusa
Acacia
xanthine oxidase
Xanthine Oxidase
Phytochemicals
allopurinol
phytopharmaceuticals
heartwood
Allopurinol
inhibitory concentration 50
extracts
reversed-phase liquid chromatography
Column chromatography
Fabaceae
medicinal plants
Taiwan
Liquid chromatography
chromatography
Reverse-Phase Chromatography
Medicinal Plants

Keywords

  • Acacia confusa
  • Extracts
  • Melanoxetin
  • Okanin
  • Xanthine oxidase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Inhibition of xanthine oxidase by acacia confusa extracts and their phytochemicals. / Tung, Yu Tang; Chang, Shang Tzen.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 2, 27.01.2010, p. 781-786.

Research output: Contribution to journalArticle

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