Inhibition of specific IgE response in vivo by allergen-gene transfer

Ching Hsiang Hsu, Kaw Yan Chua, Mi Hua Tao, Shau Ku Huang, Kue Hsiung Hsieh

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

DNA immunization has been an attractive approach in altering the host immune response to antigen. To examine the utility of DNA immunization in allergic response, we examined the in vivo efficacy of an 'allergen-gene immunization' approach in the modulation of allergen-specific IgE responses in mice. Our results showed first that i.m. injection of a gene construct (pCMVD) containing an important house dust mite allergen gene (Dermatophagoides pteronyssinus group 5 allergen; Der p 5) results in the induction of Der p 5-specific IgG antibodies, but not IgE antibody. We next examined the effect of transduced allergen gene on the expression of specific IgE response in mice after i.p. challenge with recombinant Der p 5 (rDer p 5). Both vector (mock) control- and pCMVD-treated mice were i.p. sensitized with rDer p 5 at 3 weeks after injection of gene construct. Results showed that there is a 90% reduction in the level of specific IgE in pCMVD-treated mice when compared with mock-treated mice. Furthermore, the suppression of specific IgE response can be adoptively transferred with CD8+ T cells from pCMVD-treated mice and such inhibition is in an antigen-specific manner, since the level of specific IgE to an irrelevant allergen, Der p 1, remained unchanged in comparison to that of the mock-treated group. In addition, Der p 5-specific CD8+ T cells could produce high levels of IFN-γ which probably inhibit allergen-specific IgE responses. Taken together, our results suggest that allergen-gene transfer is effective in the modulation of allergen-specific IgE responses and may provide a novel therapeutic approach.

Original languageEnglish
Pages (from-to)1405-1411
Number of pages7
JournalInternational Immunology
Volume8
Issue number9
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

Fingerprint

Allergens
Immunoglobulin E
Genes
Immunization
Dermatophagoides pteronyssinus
Dermatophagoides Antigens
T-Lymphocytes
Injections
Antibodies
DNA
Histocompatibility Antigens Class II
Immunoglobulin G
Dermatophagoides pteronyssinus antigen p 5
Gene Expression
Antigens

Keywords

  • Allergy
  • DNA immunization
  • IFN-γ
  • Immunotherapy
  • Mite allergen

ASJC Scopus subject areas

  • Immunology

Cite this

Inhibition of specific IgE response in vivo by allergen-gene transfer. / Hsu, Ching Hsiang; Chua, Kaw Yan; Tao, Mi Hua; Huang, Shau Ku; Hsieh, Kue Hsiung.

In: International Immunology, Vol. 8, No. 9, 01.01.1996, p. 1405-1411.

Research output: Contribution to journalArticle

Hsu, Ching Hsiang ; Chua, Kaw Yan ; Tao, Mi Hua ; Huang, Shau Ku ; Hsieh, Kue Hsiung. / Inhibition of specific IgE response in vivo by allergen-gene transfer. In: International Immunology. 1996 ; Vol. 8, No. 9. pp. 1405-1411.
@article{679e2e3f974641db98693417c7ad4039,
title = "Inhibition of specific IgE response in vivo by allergen-gene transfer",
abstract = "DNA immunization has been an attractive approach in altering the host immune response to antigen. To examine the utility of DNA immunization in allergic response, we examined the in vivo efficacy of an 'allergen-gene immunization' approach in the modulation of allergen-specific IgE responses in mice. Our results showed first that i.m. injection of a gene construct (pCMVD) containing an important house dust mite allergen gene (Dermatophagoides pteronyssinus group 5 allergen; Der p 5) results in the induction of Der p 5-specific IgG antibodies, but not IgE antibody. We next examined the effect of transduced allergen gene on the expression of specific IgE response in mice after i.p. challenge with recombinant Der p 5 (rDer p 5). Both vector (mock) control- and pCMVD-treated mice were i.p. sensitized with rDer p 5 at 3 weeks after injection of gene construct. Results showed that there is a 90{\%} reduction in the level of specific IgE in pCMVD-treated mice when compared with mock-treated mice. Furthermore, the suppression of specific IgE response can be adoptively transferred with CD8+ T cells from pCMVD-treated mice and such inhibition is in an antigen-specific manner, since the level of specific IgE to an irrelevant allergen, Der p 1, remained unchanged in comparison to that of the mock-treated group. In addition, Der p 5-specific CD8+ T cells could produce high levels of IFN-γ which probably inhibit allergen-specific IgE responses. Taken together, our results suggest that allergen-gene transfer is effective in the modulation of allergen-specific IgE responses and may provide a novel therapeutic approach.",
keywords = "Allergy, DNA immunization, IFN-γ, Immunotherapy, Mite allergen",
author = "Hsu, {Ching Hsiang} and Chua, {Kaw Yan} and Tao, {Mi Hua} and Huang, {Shau Ku} and Hsieh, {Kue Hsiung}",
year = "1996",
month = "1",
day = "1",
doi = "10.1093/intimm/8.9.1405",
language = "English",
volume = "8",
pages = "1405--1411",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Inhibition of specific IgE response in vivo by allergen-gene transfer

AU - Hsu, Ching Hsiang

AU - Chua, Kaw Yan

AU - Tao, Mi Hua

AU - Huang, Shau Ku

AU - Hsieh, Kue Hsiung

PY - 1996/1/1

Y1 - 1996/1/1

N2 - DNA immunization has been an attractive approach in altering the host immune response to antigen. To examine the utility of DNA immunization in allergic response, we examined the in vivo efficacy of an 'allergen-gene immunization' approach in the modulation of allergen-specific IgE responses in mice. Our results showed first that i.m. injection of a gene construct (pCMVD) containing an important house dust mite allergen gene (Dermatophagoides pteronyssinus group 5 allergen; Der p 5) results in the induction of Der p 5-specific IgG antibodies, but not IgE antibody. We next examined the effect of transduced allergen gene on the expression of specific IgE response in mice after i.p. challenge with recombinant Der p 5 (rDer p 5). Both vector (mock) control- and pCMVD-treated mice were i.p. sensitized with rDer p 5 at 3 weeks after injection of gene construct. Results showed that there is a 90% reduction in the level of specific IgE in pCMVD-treated mice when compared with mock-treated mice. Furthermore, the suppression of specific IgE response can be adoptively transferred with CD8+ T cells from pCMVD-treated mice and such inhibition is in an antigen-specific manner, since the level of specific IgE to an irrelevant allergen, Der p 1, remained unchanged in comparison to that of the mock-treated group. In addition, Der p 5-specific CD8+ T cells could produce high levels of IFN-γ which probably inhibit allergen-specific IgE responses. Taken together, our results suggest that allergen-gene transfer is effective in the modulation of allergen-specific IgE responses and may provide a novel therapeutic approach.

AB - DNA immunization has been an attractive approach in altering the host immune response to antigen. To examine the utility of DNA immunization in allergic response, we examined the in vivo efficacy of an 'allergen-gene immunization' approach in the modulation of allergen-specific IgE responses in mice. Our results showed first that i.m. injection of a gene construct (pCMVD) containing an important house dust mite allergen gene (Dermatophagoides pteronyssinus group 5 allergen; Der p 5) results in the induction of Der p 5-specific IgG antibodies, but not IgE antibody. We next examined the effect of transduced allergen gene on the expression of specific IgE response in mice after i.p. challenge with recombinant Der p 5 (rDer p 5). Both vector (mock) control- and pCMVD-treated mice were i.p. sensitized with rDer p 5 at 3 weeks after injection of gene construct. Results showed that there is a 90% reduction in the level of specific IgE in pCMVD-treated mice when compared with mock-treated mice. Furthermore, the suppression of specific IgE response can be adoptively transferred with CD8+ T cells from pCMVD-treated mice and such inhibition is in an antigen-specific manner, since the level of specific IgE to an irrelevant allergen, Der p 1, remained unchanged in comparison to that of the mock-treated group. In addition, Der p 5-specific CD8+ T cells could produce high levels of IFN-γ which probably inhibit allergen-specific IgE responses. Taken together, our results suggest that allergen-gene transfer is effective in the modulation of allergen-specific IgE responses and may provide a novel therapeutic approach.

KW - Allergy

KW - DNA immunization

KW - IFN-γ

KW - Immunotherapy

KW - Mite allergen

UR - http://www.scopus.com/inward/record.url?scp=0029818390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029818390&partnerID=8YFLogxK

U2 - 10.1093/intimm/8.9.1405

DO - 10.1093/intimm/8.9.1405

M3 - Article

C2 - 8921418

AN - SCOPUS:0029818390

VL - 8

SP - 1405

EP - 1411

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 9

ER -