Inhibition of Ras-mediated cell proliferation by benzyloxybenzaldehyde.

Shiow Lin Pan, Jih Hwa Guh, Ying Wen Huang, Ya Ling Chang, Chiung Yun Chang, Li Jiau Huang, Sheng Chu Kuo, Che Ming Teng

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11 Citations (Scopus)

Abstract

Excessive proliferation of vascular smooth muscle cells (VSMC) in the intima is an important etiologic factor in vascular proliferative disorders such as atherosclerosis and restenosis after balloon angioplasty. Therefore, control of VSMC growth may be a suitable therapeutic intervention in vascular proliferative disorders. In the present work, we have studied the 2-benzyloxybenzaldehyde (CCY1a)-mediated antiproliferative effect and its mechanisms of action. CCY1a inhibited serum-induced VSMC proliferation in a concentration-dependent manner, as demonstrated using [(3)H]thymidine incorporation and MTT assays; the IC(50) values were calculated to be 7.0 x 10(-6) and 1.2 x 10(-5) M, respectively. Furthermore, it also significantly suppressed serum-induced progression of the cell cycle, as shown by flow cytometric analysis. CCY1a as well as PD98059 almost completely abolished serum-induced activation of p42/44 mitogen-activated protein kinase (MAPK), the downstream effectors of c-fos and c-jun mRNA expression and activator protein-1 (AP-1) DNA binding activity, suggesting the central roles of these signaling cascades. Interestingly, CCY1a also effectively blocked serum-induced Ikappa B-alpha phosphorylation, Ikappa B-alpha degradation and nuclear factor-kappa B (NF-kappa B) binding activity. Based on these observations, we examined the effect of CCY1a on serum-mediated Ras activity, an upstream regulator of the above signaling events. The data demonstrated a marked inhibition of Ras activation by CCY1a. We conclude that CCY1a blocks cell proliferation via inhibition of the upstream effector of Ras and downstream events, including p42/44 MAPK activation and c-fos and c-jun mRNA expression, as well as NF-kappa B and AP-1 DNA binding activities.

Original languageEnglish
Pages (from-to)622-630
Number of pages9
JournalJournal of Biomedical Science
Volume9
Issue number6 Pt 2
Publication statusPublished - Nov 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Pan, S. L., Guh, J. H., Huang, Y. W., Chang, Y. L., Chang, C. Y., Huang, L. J., Kuo, S. C., & Teng, C. M. (2002). Inhibition of Ras-mediated cell proliferation by benzyloxybenzaldehyde. Journal of Biomedical Science, 9(6 Pt 2), 622-630.