Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells

Jiun Han Chen, Junn Liang Chang, Pei Ru Chen, Yun Ju Chuang, Shih Tsang Tang, Shwu Fen Pan, Tzer Bin Lin, Kang Hua Chen, Mei Jung Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The purpose of this study was to investigate if PPAR γ plays a role in the melanogenesis. B16/F10 cells were divided into five groups: control, melanin stimulating hormone (-MSH), -MSH+retinol, -MSH+GW9662 (PPAR γ antagonist), and GW9662. Cells in the control group were cultured in the Dulbecco's modified Eagle's medium (DMEM) for 48 hrs. To initiate the melanogenesis, cells in all -MSH groups were cultured in medium containing -MSH (10 nM) for 48 hrs. Cells were treated simultaneously with retinol (5 M) in the -MSH+retinol group. Instead of retinol, GW9662 (10 M) was cocultured in the -MSH+GW9662 group. Cells in the final group were cultured in the DMEM with GW9662. All the analyses were carried out 48 hours after treatments. The -MSH was able to increase cell number, melanin production, and the activity of tyrosinase, the limiting enzyme in melanogenesis. These -MSH-induced changes were prevented either by retinol or by GW9662. Further analyses of the activities of antioxidant enzymes including glutathione, catalase, and the superoxide dismutase (SOD) showed that -MSH treatment raised the activity of SOD which was dependent on PPAR γ level. According to our results, the -MSH-induced melanogenesis was PPAR γ dependent, which also modulated the expression of SOD.

Original languageEnglish
Article number695797
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

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Melanocyte-Stimulating Hormones
Experimental Melanomas
PPAR gamma
Vitamin A
Peroxisome Proliferator-Activated Receptors
Superoxide Dismutase
Eagles
Melanins
Control Groups
Monophenol Monooxygenase
Enzymes
Catalase
Glutathione
2-chloro-5-nitrobenzanilide
Cell Count
Antioxidants
Hormones

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Chen, J. H., Chang, J. L., Chen, P. R., Chuang, Y. J., Tang, S. T., Pan, S. F., ... Chen, M. J. (2014). Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells. BioMed Research International, 2014, [695797]. https://doi.org/10.1155/2014/695797

Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells. / Chen, Jiun Han; Chang, Junn Liang; Chen, Pei Ru; Chuang, Yun Ju; Tang, Shih Tsang; Pan, Shwu Fen; Lin, Tzer Bin; Chen, Kang Hua; Chen, Mei Jung.

In: BioMed Research International, Vol. 2014, 695797, 2014.

Research output: Contribution to journalArticle

Chen, Jiun Han ; Chang, Junn Liang ; Chen, Pei Ru ; Chuang, Yun Ju ; Tang, Shih Tsang ; Pan, Shwu Fen ; Lin, Tzer Bin ; Chen, Kang Hua ; Chen, Mei Jung. / Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells. In: BioMed Research International. 2014 ; Vol. 2014.
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