Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells

Jiun Han Chen, Junn Liang Chang, Pei Ru Chen, Yun Ju Chuang, Shih Tsang Tang, Shwu Fen Pan, Tzer Bin Lin, Kang Hua Chen, Mei Jung Chen

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5 Citations (Scopus)

Abstract

The purpose of this study was to investigate if PPAR γ plays a role in the melanogenesis. B16/F10 cells were divided into five groups: control, melanin stimulating hormone (-MSH), -MSH+retinol, -MSH+GW9662 (PPAR γ antagonist), and GW9662. Cells in the control group were cultured in the Dulbecco's modified Eagle's medium (DMEM) for 48 hrs. To initiate the melanogenesis, cells in all -MSH groups were cultured in medium containing -MSH (10 nM) for 48 hrs. Cells were treated simultaneously with retinol (5 M) in the -MSH+retinol group. Instead of retinol, GW9662 (10 M) was cocultured in the -MSH+GW9662 group. Cells in the final group were cultured in the DMEM with GW9662. All the analyses were carried out 48 hours after treatments. The -MSH was able to increase cell number, melanin production, and the activity of tyrosinase, the limiting enzyme in melanogenesis. These -MSH-induced changes were prevented either by retinol or by GW9662. Further analyses of the activities of antioxidant enzymes including glutathione, catalase, and the superoxide dismutase (SOD) showed that -MSH treatment raised the activity of SOD which was dependent on PPAR γ level. According to our results, the -MSH-induced melanogenesis was PPAR γ dependent, which also modulated the expression of SOD.

Original languageEnglish
Article number695797
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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    Chen, J. H., Chang, J. L., Chen, P. R., Chuang, Y. J., Tang, S. T., Pan, S. F., Lin, T. B., Chen, K. H., & Chen, M. J. (2014). Inhibition of Peroxisome Proliferator-Activated Receptor Gamma Prevents the Melanogenesis in Murine B16/F10 Melanoma Cells. BioMed Research International, 2014, [695797]. https://doi.org/10.1155/2014/695797