Inhibition of NF-κB and metastasis in irinotecan (CPT-11)-resistant LoVo colon cancer cells by thymoquinone via JNK and p38

Ming Cheng Chen, Nien Hung Lee, Hsi Hsien Hsu, Tsung Jung Ho, Chuan Chou Tu, Ray Jade Chen, Yueh Min Lin, Vijaya Padma Viswanadha, Wei Wen Kuo, Chih Yang Huang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Clinically used chemotherapeutics can effectively eliminate most tumor cells. However, they cause unwanted side effects and result in chemoresistance. To overcome such problems, phytochemicals are now used to treat cancers by means of targeted therapy. Thymoquinone (TQ) is used to treat different cancers (including colon cancer) and is an NF-κB inhibitor. Irinotecan resistant (CPT-11-R) LoVo colon cancer cell line was previous constructed by step-wise CPT-11 challenges to un-treated parental LoVo cells and expresses EGFR/IKKα/β/NF-κB pathway. TQ resulted in reduced total and phosphorylation of IKKα/β and NF-κB and decreased metastasis in CPT-11-R cells. TQ not only reduced activity of ERK1/2 and PI3K but also activated JNK and p38. Furthermore, TQ was also found to suppress metastasis through activation of JNK and p38. Therefore, TQ suppressed metastasis through NF-κB inhibition and activation of JNK and p38 in CPT-11-R LoVo colon cancer cells.

Original languageEnglish
JournalEnvironmental Toxicology
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

irinotecan
Colonic Neoplasms
cancer
Cells
Neoplasm Metastasis
Chemical activation
Phosphorylation
Phytochemicals
Phosphatidylinositol 3-Kinases
tumor
Tumors
inhibitor
Neoplasms
thymoquinone
Cell Line

Keywords

  • CPT-11-R LoVo colon cancer cells
  • JNK
  • NF-κB
  • P38
  • Thymoquinone

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Management, Monitoring, Policy and Law

Cite this

Inhibition of NF-κB and metastasis in irinotecan (CPT-11)-resistant LoVo colon cancer cells by thymoquinone via JNK and p38. / Chen, Ming Cheng; Lee, Nien Hung; Hsu, Hsi Hsien; Ho, Tsung Jung; Tu, Chuan Chou; Chen, Ray Jade; Lin, Yueh Min; Viswanadha, Vijaya Padma; Kuo, Wei Wen; Huang, Chih Yang.

In: Environmental Toxicology, 2016.

Research output: Contribution to journalArticle

Chen, Ming Cheng ; Lee, Nien Hung ; Hsu, Hsi Hsien ; Ho, Tsung Jung ; Tu, Chuan Chou ; Chen, Ray Jade ; Lin, Yueh Min ; Viswanadha, Vijaya Padma ; Kuo, Wei Wen ; Huang, Chih Yang. / Inhibition of NF-κB and metastasis in irinotecan (CPT-11)-resistant LoVo colon cancer cells by thymoquinone via JNK and p38. In: Environmental Toxicology. 2016.
@article{a2f69f5d71144745a8aa1e950aed9c82,
title = "Inhibition of NF-κB and metastasis in irinotecan (CPT-11)-resistant LoVo colon cancer cells by thymoquinone via JNK and p38",
abstract = "Clinically used chemotherapeutics can effectively eliminate most tumor cells. However, they cause unwanted side effects and result in chemoresistance. To overcome such problems, phytochemicals are now used to treat cancers by means of targeted therapy. Thymoquinone (TQ) is used to treat different cancers (including colon cancer) and is an NF-κB inhibitor. Irinotecan resistant (CPT-11-R) LoVo colon cancer cell line was previous constructed by step-wise CPT-11 challenges to un-treated parental LoVo cells and expresses EGFR/IKKα/β/NF-κB pathway. TQ resulted in reduced total and phosphorylation of IKKα/β and NF-κB and decreased metastasis in CPT-11-R cells. TQ not only reduced activity of ERK1/2 and PI3K but also activated JNK and p38. Furthermore, TQ was also found to suppress metastasis through activation of JNK and p38. Therefore, TQ suppressed metastasis through NF-κB inhibition and activation of JNK and p38 in CPT-11-R LoVo colon cancer cells.",
keywords = "CPT-11-R LoVo colon cancer cells, JNK, NF-κB, P38, Thymoquinone",
author = "Chen, {Ming Cheng} and Lee, {Nien Hung} and Hsu, {Hsi Hsien} and Ho, {Tsung Jung} and Tu, {Chuan Chou} and Chen, {Ray Jade} and Lin, {Yueh Min} and Viswanadha, {Vijaya Padma} and Kuo, {Wei Wen} and Huang, {Chih Yang}",
year = "2016",
doi = "10.1002/tox.22268",
language = "English",
journal = "Environmental Toxicology",
issn = "1520-4081",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Inhibition of NF-κB and metastasis in irinotecan (CPT-11)-resistant LoVo colon cancer cells by thymoquinone via JNK and p38

AU - Chen, Ming Cheng

AU - Lee, Nien Hung

AU - Hsu, Hsi Hsien

AU - Ho, Tsung Jung

AU - Tu, Chuan Chou

AU - Chen, Ray Jade

AU - Lin, Yueh Min

AU - Viswanadha, Vijaya Padma

AU - Kuo, Wei Wen

AU - Huang, Chih Yang

PY - 2016

Y1 - 2016

N2 - Clinically used chemotherapeutics can effectively eliminate most tumor cells. However, they cause unwanted side effects and result in chemoresistance. To overcome such problems, phytochemicals are now used to treat cancers by means of targeted therapy. Thymoquinone (TQ) is used to treat different cancers (including colon cancer) and is an NF-κB inhibitor. Irinotecan resistant (CPT-11-R) LoVo colon cancer cell line was previous constructed by step-wise CPT-11 challenges to un-treated parental LoVo cells and expresses EGFR/IKKα/β/NF-κB pathway. TQ resulted in reduced total and phosphorylation of IKKα/β and NF-κB and decreased metastasis in CPT-11-R cells. TQ not only reduced activity of ERK1/2 and PI3K but also activated JNK and p38. Furthermore, TQ was also found to suppress metastasis through activation of JNK and p38. Therefore, TQ suppressed metastasis through NF-κB inhibition and activation of JNK and p38 in CPT-11-R LoVo colon cancer cells.

AB - Clinically used chemotherapeutics can effectively eliminate most tumor cells. However, they cause unwanted side effects and result in chemoresistance. To overcome such problems, phytochemicals are now used to treat cancers by means of targeted therapy. Thymoquinone (TQ) is used to treat different cancers (including colon cancer) and is an NF-κB inhibitor. Irinotecan resistant (CPT-11-R) LoVo colon cancer cell line was previous constructed by step-wise CPT-11 challenges to un-treated parental LoVo cells and expresses EGFR/IKKα/β/NF-κB pathway. TQ resulted in reduced total and phosphorylation of IKKα/β and NF-κB and decreased metastasis in CPT-11-R cells. TQ not only reduced activity of ERK1/2 and PI3K but also activated JNK and p38. Furthermore, TQ was also found to suppress metastasis through activation of JNK and p38. Therefore, TQ suppressed metastasis through NF-κB inhibition and activation of JNK and p38 in CPT-11-R LoVo colon cancer cells.

KW - CPT-11-R LoVo colon cancer cells

KW - JNK

KW - NF-κB

KW - P38

KW - Thymoquinone

UR - http://www.scopus.com/inward/record.url?scp=84963800268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84963800268&partnerID=8YFLogxK

U2 - 10.1002/tox.22268

DO - 10.1002/tox.22268

M3 - Article

C2 - 27060453

AN - SCOPUS:84963800268

JO - Environmental Toxicology

JF - Environmental Toxicology

SN - 1520-4081

ER -