Abstract
The expression of phospholipase D (PLD) isoenzymes in neutrophils was investigated using reverse transcription-polymerase chain reaction analysis. Amplification products of predicted size were obtained from rat neutrophils with nucleotide sequences corresponding to PLD1a and PLD2. 1-(3′,4′-Dimethoxybenzyl)-6,7-dichloroisoquinoline (DMDI) inhibited the formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated PLD activation in rat neutrophils. The underlying cellular signaling mechanism of DMDI inhibition was investigated. The fMLP-induced protein tyrosine phosphorylation and the membrane translocation of ADP-ribosylation factor (ARF) and Rho A in neutrophils was attenuated by DMDI in a concentration-dependent manner. However, neither the membrane association of protein kinase C-α and -β isoenzymes in fMLP-stimulated cells nor the GTPγS- and phorbol 12-myristate 13-acetate-stimulated membrane translocation of ARF and Rho A in a cell-free system was affected significantly by DMDI. These results indicate that the expression of PLD1a and PLD2 mRNA in neutrophils. Attenuation of protein tyrosine phosphorylation and the membrane association of ARF and Rho A probably play a concerted role in the inhibition of PLD by DMDI in rat neutrophils in response to fMLP.
| Original language | English |
|---|---|
| Pages (from-to) | 191-198 |
| Number of pages | 8 |
| Journal | Biochimica et Biophysica Acta - General Subjects |
| Volume | 1620 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - Mar 17 2003 |
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Keywords
- ADP-ribosylation factor
- Neutrophil
- Phospholipase D1a
- Phospholipase D2
- Protein tyrosine phosphorylation
- Rho A
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Inhibition of formyl-methionyl-leucyl-phenylalanine-stimulated phospholipase D activation in rat neutrophils by the synthetic isoquinoline DMDI. / Chang, Ling Chu; Chen, Chi Ming; Wang, Jih Pyang.
In: Biochimica et Biophysica Acta - General Subjects, Vol. 1620, No. 1-3, 17.03.2003, p. 191-198.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of formyl-methionyl-leucyl-phenylalanine-stimulated phospholipase D activation in rat neutrophils by the synthetic isoquinoline DMDI
AU - Chang, Ling Chu
AU - Chen, Chi Ming
AU - Wang, Jih Pyang
PY - 2003/3/17
Y1 - 2003/3/17
N2 - The expression of phospholipase D (PLD) isoenzymes in neutrophils was investigated using reverse transcription-polymerase chain reaction analysis. Amplification products of predicted size were obtained from rat neutrophils with nucleotide sequences corresponding to PLD1a and PLD2. 1-(3′,4′-Dimethoxybenzyl)-6,7-dichloroisoquinoline (DMDI) inhibited the formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated PLD activation in rat neutrophils. The underlying cellular signaling mechanism of DMDI inhibition was investigated. The fMLP-induced protein tyrosine phosphorylation and the membrane translocation of ADP-ribosylation factor (ARF) and Rho A in neutrophils was attenuated by DMDI in a concentration-dependent manner. However, neither the membrane association of protein kinase C-α and -β isoenzymes in fMLP-stimulated cells nor the GTPγS- and phorbol 12-myristate 13-acetate-stimulated membrane translocation of ARF and Rho A in a cell-free system was affected significantly by DMDI. These results indicate that the expression of PLD1a and PLD2 mRNA in neutrophils. Attenuation of protein tyrosine phosphorylation and the membrane association of ARF and Rho A probably play a concerted role in the inhibition of PLD by DMDI in rat neutrophils in response to fMLP.
AB - The expression of phospholipase D (PLD) isoenzymes in neutrophils was investigated using reverse transcription-polymerase chain reaction analysis. Amplification products of predicted size were obtained from rat neutrophils with nucleotide sequences corresponding to PLD1a and PLD2. 1-(3′,4′-Dimethoxybenzyl)-6,7-dichloroisoquinoline (DMDI) inhibited the formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated PLD activation in rat neutrophils. The underlying cellular signaling mechanism of DMDI inhibition was investigated. The fMLP-induced protein tyrosine phosphorylation and the membrane translocation of ADP-ribosylation factor (ARF) and Rho A in neutrophils was attenuated by DMDI in a concentration-dependent manner. However, neither the membrane association of protein kinase C-α and -β isoenzymes in fMLP-stimulated cells nor the GTPγS- and phorbol 12-myristate 13-acetate-stimulated membrane translocation of ARF and Rho A in a cell-free system was affected significantly by DMDI. These results indicate that the expression of PLD1a and PLD2 mRNA in neutrophils. Attenuation of protein tyrosine phosphorylation and the membrane association of ARF and Rho A probably play a concerted role in the inhibition of PLD by DMDI in rat neutrophils in response to fMLP.
KW - ADP-ribosylation factor
KW - Neutrophil
KW - Phospholipase D1a
KW - Phospholipase D2
KW - Protein tyrosine phosphorylation
KW - Rho A
UR - http://www.scopus.com/inward/record.url?scp=12244258696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12244258696&partnerID=8YFLogxK
U2 - 10.1016/S0304-4165(02)00532-9
DO - 10.1016/S0304-4165(02)00532-9
M3 - Article
VL - 1620
SP - 191
EP - 198
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 1-3
ER -