Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate

Li Kwan Chang, Ta Tung Wei, Ya Fang Chiu, Chao Ping Tung, Jian Ying Chuang, Shang K. Hung, Ching Li, Shih Tung Liu

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

(-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.

Original languageEnglish
Pages (from-to)1062-1068
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number4
DOIs
Publication statusPublished - Feb 21 2003
Externally publishedYes

Fingerprint

Human Herpesvirus 4
Viruses
Microarrays
Immediate-Early Genes
Flow cytometry
Tea
Transcription
Indirect Fluorescent Antibody Technique
Oligonucleotide Array Sequence Analysis
Transfection
Tumors
Flow Cytometry
Genes
DNA
epigallocatechin gallate
Neoplasms
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate. / Chang, Li Kwan; Wei, Ta Tung; Chiu, Ya Fang; Tung, Chao Ping; Chuang, Jian Ying; Hung, Shang K.; Li, Ching; Liu, Shih Tung.

In: Biochemical and Biophysical Research Communications, Vol. 301, No. 4, 21.02.2003, p. 1062-1068.

Research output: Contribution to journalArticle

Chang, Li Kwan ; Wei, Ta Tung ; Chiu, Ya Fang ; Tung, Chao Ping ; Chuang, Jian Ying ; Hung, Shang K. ; Li, Ching ; Liu, Shih Tung. / Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 301, No. 4. pp. 1062-1068.
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