Inhibition of eleven mutagens by various tea extracts, (-)epigallocatechin-3-gallate, gallic acid and caffeine

Tzyh Chyuan Hour, Yu Chih Liang, Iou Sen Chu, Jen Kun Lin

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The antimutagenic properties of various tea extracts (green tea, pauchong tea, oolong tea and black tea) and their components including (-)-epigallocatechin-3-gallate (EGCG), gallic acid and caffeine were examined by the Ames test. The antimutagenic activity of the green tea extract against N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), folpet and monocrotophos was greater than those of pouchong, oolong and black tea extracts. The antimutagenic effects of tea extracts against 2-acetylaminofluorene (AAF) decreased as follows: oolong tea >pauchong tea >black tea >green tea. Furthermore, black tea showed a greater antimutagenic activity against benzo[a]pyrene (BP). The pauchong tea showed a stronger inhibitory effect against 9-aminoacridine (9AA) and aflatoxin B1 (AFB1) than other tea extracts. EGCG markedly suppressed the direct-acting mutagenicity of MNNG, N-nitroso-N-methylurea (MNU), captan, and folpet which were alkylating agents and fungicides. Similarly, gallic acid, the major component of black tea strongly inhibited the mutagenicity of 9AA, and moderately inhibited the mutagenicity of MNNG and folpet. The caffeine was less active. EGCG and gallic acid perhaps could act as nucleophiles to scavenge the electrophilic mutagens. Taken together, these results suggest that formation of different metabolites during various stages of tea fermentation may affect antimutagenic potencies against different types of chemical mutagens. Copyright (C) 1999 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)569-579
Number of pages11
JournalFood and Chemical Toxicology
Volume37
Issue number6
DOIs
Publication statusPublished - Jun 1999
Externally publishedYes

Fingerprint

tea (beverage)
Gallic Acid
epigallocatechin
Mutagens
Tea
caffeine
Caffeine
gallic acid
black tea
antimutagenic activity
folpet
extracts
mutagenicity
green tea
monocrotophos
Ames test
captan
Aminacrine
aflatoxin B1
Methylnitronitrosoguanidine

Keywords

  • Alkylating agent
  • Ames test
  • EGCG
  • MNNG
  • Mutagenicity

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Inhibition of eleven mutagens by various tea extracts, (-)epigallocatechin-3-gallate, gallic acid and caffeine. / Hour, Tzyh Chyuan; Liang, Yu Chih; Chu, Iou Sen; Lin, Jen Kun.

In: Food and Chemical Toxicology, Vol. 37, No. 6, 06.1999, p. 569-579.

Research output: Contribution to journalArticle

@article{ef729545e670472a80af45241b72a6fa,
title = "Inhibition of eleven mutagens by various tea extracts, (-)epigallocatechin-3-gallate, gallic acid and caffeine",
abstract = "The antimutagenic properties of various tea extracts (green tea, pauchong tea, oolong tea and black tea) and their components including (-)-epigallocatechin-3-gallate (EGCG), gallic acid and caffeine were examined by the Ames test. The antimutagenic activity of the green tea extract against N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), folpet and monocrotophos was greater than those of pouchong, oolong and black tea extracts. The antimutagenic effects of tea extracts against 2-acetylaminofluorene (AAF) decreased as follows: oolong tea >pauchong tea >black tea >green tea. Furthermore, black tea showed a greater antimutagenic activity against benzo[a]pyrene (BP). The pauchong tea showed a stronger inhibitory effect against 9-aminoacridine (9AA) and aflatoxin B1 (AFB1) than other tea extracts. EGCG markedly suppressed the direct-acting mutagenicity of MNNG, N-nitroso-N-methylurea (MNU), captan, and folpet which were alkylating agents and fungicides. Similarly, gallic acid, the major component of black tea strongly inhibited the mutagenicity of 9AA, and moderately inhibited the mutagenicity of MNNG and folpet. The caffeine was less active. EGCG and gallic acid perhaps could act as nucleophiles to scavenge the electrophilic mutagens. Taken together, these results suggest that formation of different metabolites during various stages of tea fermentation may affect antimutagenic potencies against different types of chemical mutagens. Copyright (C) 1999 Elsevier Science Ltd.",
keywords = "Alkylating agent, Ames test, EGCG, MNNG, Mutagenicity",
author = "Hour, {Tzyh Chyuan} and Liang, {Yu Chih} and Chu, {Iou Sen} and Lin, {Jen Kun}",
year = "1999",
month = "6",
doi = "10.1016/S0278-6915(99)00031-9",
language = "English",
volume = "37",
pages = "569--579",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Inhibition of eleven mutagens by various tea extracts, (-)epigallocatechin-3-gallate, gallic acid and caffeine

AU - Hour, Tzyh Chyuan

AU - Liang, Yu Chih

AU - Chu, Iou Sen

AU - Lin, Jen Kun

PY - 1999/6

Y1 - 1999/6

N2 - The antimutagenic properties of various tea extracts (green tea, pauchong tea, oolong tea and black tea) and their components including (-)-epigallocatechin-3-gallate (EGCG), gallic acid and caffeine were examined by the Ames test. The antimutagenic activity of the green tea extract against N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), folpet and monocrotophos was greater than those of pouchong, oolong and black tea extracts. The antimutagenic effects of tea extracts against 2-acetylaminofluorene (AAF) decreased as follows: oolong tea >pauchong tea >black tea >green tea. Furthermore, black tea showed a greater antimutagenic activity against benzo[a]pyrene (BP). The pauchong tea showed a stronger inhibitory effect against 9-aminoacridine (9AA) and aflatoxin B1 (AFB1) than other tea extracts. EGCG markedly suppressed the direct-acting mutagenicity of MNNG, N-nitroso-N-methylurea (MNU), captan, and folpet which were alkylating agents and fungicides. Similarly, gallic acid, the major component of black tea strongly inhibited the mutagenicity of 9AA, and moderately inhibited the mutagenicity of MNNG and folpet. The caffeine was less active. EGCG and gallic acid perhaps could act as nucleophiles to scavenge the electrophilic mutagens. Taken together, these results suggest that formation of different metabolites during various stages of tea fermentation may affect antimutagenic potencies against different types of chemical mutagens. Copyright (C) 1999 Elsevier Science Ltd.

AB - The antimutagenic properties of various tea extracts (green tea, pauchong tea, oolong tea and black tea) and their components including (-)-epigallocatechin-3-gallate (EGCG), gallic acid and caffeine were examined by the Ames test. The antimutagenic activity of the green tea extract against N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), folpet and monocrotophos was greater than those of pouchong, oolong and black tea extracts. The antimutagenic effects of tea extracts against 2-acetylaminofluorene (AAF) decreased as follows: oolong tea >pauchong tea >black tea >green tea. Furthermore, black tea showed a greater antimutagenic activity against benzo[a]pyrene (BP). The pauchong tea showed a stronger inhibitory effect against 9-aminoacridine (9AA) and aflatoxin B1 (AFB1) than other tea extracts. EGCG markedly suppressed the direct-acting mutagenicity of MNNG, N-nitroso-N-methylurea (MNU), captan, and folpet which were alkylating agents and fungicides. Similarly, gallic acid, the major component of black tea strongly inhibited the mutagenicity of 9AA, and moderately inhibited the mutagenicity of MNNG and folpet. The caffeine was less active. EGCG and gallic acid perhaps could act as nucleophiles to scavenge the electrophilic mutagens. Taken together, these results suggest that formation of different metabolites during various stages of tea fermentation may affect antimutagenic potencies against different types of chemical mutagens. Copyright (C) 1999 Elsevier Science Ltd.

KW - Alkylating agent

KW - Ames test

KW - EGCG

KW - MNNG

KW - Mutagenicity

UR - http://www.scopus.com/inward/record.url?scp=0032821789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032821789&partnerID=8YFLogxK

U2 - 10.1016/S0278-6915(99)00031-9

DO - 10.1016/S0278-6915(99)00031-9

M3 - Article

C2 - 10478825

AN - SCOPUS:0032821789

VL - 37

SP - 569

EP - 579

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

IS - 6

ER -