Abstract
Cresol is a well-known environmental pollutant, toluene metabolite, uremic toxicant and accidental poisoning product. Formocresol, a preparation of formalin and cresol, is also used as a root canal medicament and for pulpotomy of primary teeth. However, little is known about its effect on cardiovascular system. In this study, m-cresol inhibited the AA-induced platelet aggregation by 43-97% at concentrations ranging from 0.25 to 1 mM. Collagen-induced platelet aggregation was also inhibited by 0.25-1 mM of m-cresol by 47-98%. Accordingly, o-cresol (0.1-0.5 mM) also inhibited the AA-induced platelet aggregation by 46-96% and the collagen-induced platelet aggregation by 35-88% at concentrations of 0.1-1 mM. AA- and collagen-induced platelet thromboxane B 2 (TXB 2) production was inhibited by even 0.1 mM of m-cresol with 88 and 54% of inhibition, respectively. The o-cresol (0.1 mM) also inhibited the AA- and collagen-induced platelet TXB 2 production with 91 and 97% respectively. Although m- and o-cresol (2 production. The m-cresol (2 and 5 mM) inhibited the COX-1 activity by 55-99%, but showed little effect on COX-2 enzyme activity. Moreover, o-cresol (0.5 and 1 mM) inhibited the COX-1 activity by 40-95%. COX-2 enzyme activity was inhibited by 68% at a concentration of 5 mM o-cresol. These results indicate that acute cresol-poisoning, direct root canal medication with formocresol or long-term occupational exposure to cresol and toluene may potentially suppress blood clot formation and lead to tissue hemorrhage via inhibition of platelet aggregation, TXB 2 production and COX enzyme activity.
| Original language | English |
|---|---|
| Pages (from-to) | 95-104 |
| Number of pages | 10 |
| Journal | Toxicology |
| Volume | 208 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 1 2005 |
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Keywords
- Cresol
- Cyclooxygenase
- Forensic science
- Hemorrhage
- Platelet aggregation
- Thromboxane
ASJC Scopus subject areas
- Toxicology
Cite this
Inhibition of cyclooxygenase activity, platelet aggregation and thromboxane B 2 production by two environmental toxicants : m- and o-cresol. / Chan, Chiu Po; Yuan-Soon, Ho; Wang, Ying Jen; Lan, Wan Hong; Chen, Lin I.; Chen, Yi Jane; Lin, Bor Ru; Chang, Mei Chi; Jeng, Jiiang Huei.
In: Toxicology, Vol. 208, No. 1, 01.03.2005, p. 95-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of cyclooxygenase activity, platelet aggregation and thromboxane B 2 production by two environmental toxicants
T2 - m- and o-cresol
AU - Chan, Chiu Po
AU - Yuan-Soon, Ho
AU - Wang, Ying Jen
AU - Lan, Wan Hong
AU - Chen, Lin I.
AU - Chen, Yi Jane
AU - Lin, Bor Ru
AU - Chang, Mei Chi
AU - Jeng, Jiiang Huei
PY - 2005/3/1
Y1 - 2005/3/1
N2 - Cresol is a well-known environmental pollutant, toluene metabolite, uremic toxicant and accidental poisoning product. Formocresol, a preparation of formalin and cresol, is also used as a root canal medicament and for pulpotomy of primary teeth. However, little is known about its effect on cardiovascular system. In this study, m-cresol inhibited the AA-induced platelet aggregation by 43-97% at concentrations ranging from 0.25 to 1 mM. Collagen-induced platelet aggregation was also inhibited by 0.25-1 mM of m-cresol by 47-98%. Accordingly, o-cresol (0.1-0.5 mM) also inhibited the AA-induced platelet aggregation by 46-96% and the collagen-induced platelet aggregation by 35-88% at concentrations of 0.1-1 mM. AA- and collagen-induced platelet thromboxane B 2 (TXB 2) production was inhibited by even 0.1 mM of m-cresol with 88 and 54% of inhibition, respectively. The o-cresol (0.1 mM) also inhibited the AA- and collagen-induced platelet TXB 2 production with 91 and 97% respectively. Although m- and o-cresol (2 production. The m-cresol (2 and 5 mM) inhibited the COX-1 activity by 55-99%, but showed little effect on COX-2 enzyme activity. Moreover, o-cresol (0.5 and 1 mM) inhibited the COX-1 activity by 40-95%. COX-2 enzyme activity was inhibited by 68% at a concentration of 5 mM o-cresol. These results indicate that acute cresol-poisoning, direct root canal medication with formocresol or long-term occupational exposure to cresol and toluene may potentially suppress blood clot formation and lead to tissue hemorrhage via inhibition of platelet aggregation, TXB 2 production and COX enzyme activity.
AB - Cresol is a well-known environmental pollutant, toluene metabolite, uremic toxicant and accidental poisoning product. Formocresol, a preparation of formalin and cresol, is also used as a root canal medicament and for pulpotomy of primary teeth. However, little is known about its effect on cardiovascular system. In this study, m-cresol inhibited the AA-induced platelet aggregation by 43-97% at concentrations ranging from 0.25 to 1 mM. Collagen-induced platelet aggregation was also inhibited by 0.25-1 mM of m-cresol by 47-98%. Accordingly, o-cresol (0.1-0.5 mM) also inhibited the AA-induced platelet aggregation by 46-96% and the collagen-induced platelet aggregation by 35-88% at concentrations of 0.1-1 mM. AA- and collagen-induced platelet thromboxane B 2 (TXB 2) production was inhibited by even 0.1 mM of m-cresol with 88 and 54% of inhibition, respectively. The o-cresol (0.1 mM) also inhibited the AA- and collagen-induced platelet TXB 2 production with 91 and 97% respectively. Although m- and o-cresol (2 production. The m-cresol (2 and 5 mM) inhibited the COX-1 activity by 55-99%, but showed little effect on COX-2 enzyme activity. Moreover, o-cresol (0.5 and 1 mM) inhibited the COX-1 activity by 40-95%. COX-2 enzyme activity was inhibited by 68% at a concentration of 5 mM o-cresol. These results indicate that acute cresol-poisoning, direct root canal medication with formocresol or long-term occupational exposure to cresol and toluene may potentially suppress blood clot formation and lead to tissue hemorrhage via inhibition of platelet aggregation, TXB 2 production and COX enzyme activity.
KW - Cresol
KW - Cyclooxygenase
KW - Forensic science
KW - Hemorrhage
KW - Platelet aggregation
KW - Thromboxane
UR - http://www.scopus.com/inward/record.url?scp=12444310802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12444310802&partnerID=8YFLogxK
U2 - 10.1016/j.tox.2004.11.010
DO - 10.1016/j.tox.2004.11.010
M3 - Article
C2 - 15664436
AN - SCOPUS:12444310802
VL - 208
SP - 95
EP - 104
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 1
ER -