Inhibition of cyclic strain-induced endothelin-1 secretion by baicalein in human umbilical vein endothelial cells

Hsi Hsien Chen, Hong Jye Hong, Yu Hsiang Chou, Tzu-Hurng Cheng, Jin Jer Chen, Heng Lin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Baicalein is a flavonoid extracted from the root of Scutellaria baicalensis Georgi, a medicinal plant traditionally used in Oriental medicine. Among its biological activities, baicalein has been reported to exhibit antioxidant effects. Endothelin-1 (ET-1) is a potent vasopressor synthesized by endothelial cells both in culture and in vivo. The aims of this study were to test the hypothesis that baicalein may alter strain-induced ET-1 secretion and to identify the putative underlying signaling pathways in endothelial cells. We show that baicalein inhibited strain-induced ET-1 secretion. Baicalein also inhibited strain-increased reactive oxygen species (ROS) formation and the extracellular signal-regulated kinases (ERK) phosphorylation. Using a reporter gene assay, baicalein and the antioxidant Trolox also attenuated the strain-stimulated activator protein-1 (AP-1) reporter activity. We conclude that baicalein inhibits strain-induced ET-1 gene expression, partially by interfering with the ERK pathway via attenuation of ROS formation. These results highlight the molecular pathways that may contribute to the beneficial effects of baicalein in the vascular system such as stroke prevention.

Original languageEnglish
Pages (from-to)1318-1321
Number of pages4
JournalPlanta Medica
Volume72
Issue number14
DOIs
Publication statusPublished - Nov 2006

Fingerprint

endothelins
Endothelial cells
Human Umbilical Vein Endothelial Cells
Endothelin-1
secretion
mitogen-activated protein kinase
endothelial cells
reactive oxygen species
Oriental traditional medicine
Scutellaria baicalensis
Extracellular Signal-Regulated MAP Kinases
antioxidants
plant vascular system
Reactive Oxygen Species
stroke
reporter genes
Endothelial Cells
Antioxidants
East Asian Traditional Medicine
medicinal plants

Keywords

  • Baicalein
  • Endothelial cells
  • Endothelin-1
  • Extracellular signal-regulated kinases
  • Reactive oxygen species
  • Strain

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Inhibition of cyclic strain-induced endothelin-1 secretion by baicalein in human umbilical vein endothelial cells. / Chen, Hsi Hsien; Hong, Hong Jye; Chou, Yu Hsiang; Cheng, Tzu-Hurng; Chen, Jin Jer; Lin, Heng.

In: Planta Medica, Vol. 72, No. 14, 11.2006, p. 1318-1321.

Research output: Contribution to journalArticle

Chen, Hsi Hsien ; Hong, Hong Jye ; Chou, Yu Hsiang ; Cheng, Tzu-Hurng ; Chen, Jin Jer ; Lin, Heng. / Inhibition of cyclic strain-induced endothelin-1 secretion by baicalein in human umbilical vein endothelial cells. In: Planta Medica. 2006 ; Vol. 72, No. 14. pp. 1318-1321.
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