Inhibition of cell proliferation and in vitro markers of angiogenesis by indole-3-carbinol, a major indole metabolite present in cruciferous vegetables

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A variety of studies have suggested a cancer protective role of cruciferous vegetables. In the present study, we investigated the effect of indole-3-carbinol (I3C), a major indole metabolite in cruciferous vegetables, on cell proliferation and in vitro markers of angiogenesis in phorbol myristate acetate (PMA)-stimulated endothelial EA hy926 cells. The results showed that I3C inhibited the growth of EA hy926 cells in a concentration-dependent manner. The capillary-like tube formation by PMA-activated endothelial cells was significantly suppressed by I3C, and such inhibition was associated with decreased vascular endothelial growth factor (VEGF) and increased interleukin-8 (IL-8) secretion, but not with the expression of VEGF receptor-2 protein. Additionally, gelatin zymography analysis indicated that I3C suppressed activities of matrix metalloproteinases-2 (MMP-2) and MMP-9 stimulated by PMA. These results suggest that the dietary I3C may be useful in the treatment of human cancers and angiogenic diseases.

Original languageEnglish
Pages (from-to)5164-5169
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number13
DOIs
Publication statusPublished - Jun 29 2005

Fingerprint

Vegetables
Cell proliferation
indoles
Metabolites
angiogenesis
cell proliferation
vegetables
Cell Proliferation
metabolites
Tetradecanoylphorbol Acetate
acetates
Vascular Endothelial Growth Factor Receptor-2
vascular endothelial growth factor receptor-2
Matrix Metalloproteinase 2
Endothelial cells
Gelatin
gelatinase A
gelatinase B
neoplasms
Matrix Metalloproteinases

Keywords

  • Angiogenesis
  • Cruciferous vegetables
  • Indole-3-carbinol
  • Matrix metalloproteinases
  • Tube formation
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

@article{d3346038ccea4777b850b36aaa2ed647,
title = "Inhibition of cell proliferation and in vitro markers of angiogenesis by indole-3-carbinol, a major indole metabolite present in cruciferous vegetables",
abstract = "A variety of studies have suggested a cancer protective role of cruciferous vegetables. In the present study, we investigated the effect of indole-3-carbinol (I3C), a major indole metabolite in cruciferous vegetables, on cell proliferation and in vitro markers of angiogenesis in phorbol myristate acetate (PMA)-stimulated endothelial EA hy926 cells. The results showed that I3C inhibited the growth of EA hy926 cells in a concentration-dependent manner. The capillary-like tube formation by PMA-activated endothelial cells was significantly suppressed by I3C, and such inhibition was associated with decreased vascular endothelial growth factor (VEGF) and increased interleukin-8 (IL-8) secretion, but not with the expression of VEGF receptor-2 protein. Additionally, gelatin zymography analysis indicated that I3C suppressed activities of matrix metalloproteinases-2 (MMP-2) and MMP-9 stimulated by PMA. These results suggest that the dietary I3C may be useful in the treatment of human cancers and angiogenic diseases.",
keywords = "Angiogenesis, Cruciferous vegetables, Indole-3-carbinol, Matrix metalloproteinases, Tube formation, Vascular endothelial growth factor",
author = "Wu, {Hsiao Ting} and Lin, {Shyh Hsiang} and Chen, {Yue Hwa}",
year = "2005",
month = "6",
day = "29",
doi = "10.1021/jf050034w",
language = "English",
volume = "53",
pages = "5164--5169",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "13",

}

TY - JOUR

T1 - Inhibition of cell proliferation and in vitro markers of angiogenesis by indole-3-carbinol, a major indole metabolite present in cruciferous vegetables

AU - Wu, Hsiao Ting

AU - Lin, Shyh Hsiang

AU - Chen, Yue Hwa

PY - 2005/6/29

Y1 - 2005/6/29

N2 - A variety of studies have suggested a cancer protective role of cruciferous vegetables. In the present study, we investigated the effect of indole-3-carbinol (I3C), a major indole metabolite in cruciferous vegetables, on cell proliferation and in vitro markers of angiogenesis in phorbol myristate acetate (PMA)-stimulated endothelial EA hy926 cells. The results showed that I3C inhibited the growth of EA hy926 cells in a concentration-dependent manner. The capillary-like tube formation by PMA-activated endothelial cells was significantly suppressed by I3C, and such inhibition was associated with decreased vascular endothelial growth factor (VEGF) and increased interleukin-8 (IL-8) secretion, but not with the expression of VEGF receptor-2 protein. Additionally, gelatin zymography analysis indicated that I3C suppressed activities of matrix metalloproteinases-2 (MMP-2) and MMP-9 stimulated by PMA. These results suggest that the dietary I3C may be useful in the treatment of human cancers and angiogenic diseases.

AB - A variety of studies have suggested a cancer protective role of cruciferous vegetables. In the present study, we investigated the effect of indole-3-carbinol (I3C), a major indole metabolite in cruciferous vegetables, on cell proliferation and in vitro markers of angiogenesis in phorbol myristate acetate (PMA)-stimulated endothelial EA hy926 cells. The results showed that I3C inhibited the growth of EA hy926 cells in a concentration-dependent manner. The capillary-like tube formation by PMA-activated endothelial cells was significantly suppressed by I3C, and such inhibition was associated with decreased vascular endothelial growth factor (VEGF) and increased interleukin-8 (IL-8) secretion, but not with the expression of VEGF receptor-2 protein. Additionally, gelatin zymography analysis indicated that I3C suppressed activities of matrix metalloproteinases-2 (MMP-2) and MMP-9 stimulated by PMA. These results suggest that the dietary I3C may be useful in the treatment of human cancers and angiogenic diseases.

KW - Angiogenesis

KW - Cruciferous vegetables

KW - Indole-3-carbinol

KW - Matrix metalloproteinases

KW - Tube formation

KW - Vascular endothelial growth factor

UR - http://www.scopus.com/inward/record.url?scp=22344435857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22344435857&partnerID=8YFLogxK

U2 - 10.1021/jf050034w

DO - 10.1021/jf050034w

M3 - Article

C2 - 15969492

AN - SCOPUS:22344435857

VL - 53

SP - 5164

EP - 5169

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 13

ER -