Inhibition of 1,2,4-benzenetriol-generated active oxygen species and induction of phase II enzymes by green tea polyphenols

Shern Fwu Lee, Yu Chih Liang, Jen Kun Lin

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Autooxidation of polyphenolic metabolites of benzene, such as hydroquionone (HQ), catechol (CT), 1,2,4-benzenetriol (BT) and pyrogallol (PG), produced several kinds of active oxygen species (AOS). BT and PG induced DNA breaks in the absence of metal ions, especially when producing AOS such as H2O2, O2-, HOor1ΔgO2. HQ and CT did not result in double-strand DNA breaks, except when ferrous ion was added, indicating the participation of the Fenton reaction. Polyphenolic fractions isolated from green tea (GTP) exerted inhibitory effects on the autooxidation of BT and suppressive effects on H2O2 or HO generated from phenolic metabolites of benzene in the presence of S9 or an in vivo system. Additionally, although the activities of antioxidant and phase II enzymes were elevated by both GTP and phenolic metabolites of benzene, GTP counteracted the lowering GSH caused by phenolic metabolites of benzene in rat liver. The above results suggest that GTP and phenolic metabolites of benzene are antagonistic in their response to AOS, especially hydroxyl radical.

Original languageEnglish
Pages (from-to)283-301
Number of pages19
JournalChemico-Biological Interactions
Volume98
Issue number3
DOIs
Publication statusPublished - Dec 22 1995
Externally publishedYes

Fingerprint

Polyphenols
Tea
Metabolites
Benzene
Reactive Oxygen Species
Guanosine Triphosphate
Pyrogallol
Enzymes
Ions
DNA Breaks
Double-Stranded DNA Breaks
DNA
Hydroxyl Radical
Liver
Metal ions
Rats
Antioxidants
Metals
hydroxyhydroquinone
catechol

Keywords

  • Active oxygen species
  • Antioxidant
  • Green tea polyphenol
  • Phenolic metabolites of benzene

ASJC Scopus subject areas

  • Toxicology

Cite this

Inhibition of 1,2,4-benzenetriol-generated active oxygen species and induction of phase II enzymes by green tea polyphenols. / Lee, Shern Fwu; Liang, Yu Chih; Lin, Jen Kun.

In: Chemico-Biological Interactions, Vol. 98, No. 3, 22.12.1995, p. 283-301.

Research output: Contribution to journalArticle

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