Abstract
Objective: Adipose tissue–derived stem cells (ASCs) are a promising source of cells for articular cartilage regeneration. However, ASCs isolated from different adipose tissue depots have heterogeneous cell characterizations and differentiation potential when cultured in 3-dimensional (3D) niches. Design: We compared the chondrogenicity of ASCs isolated from infrapatellar fat pads (IPFPs) and subcutaneous fat pads (SCFPs) in 3D gelatin-based biomimetic matrix. Results: The IPFP-ASC-differentiated chondrocytes had higher ACAN, COL2A1, COL10, SOX6, SOX9, ChM-1, and MIA-3 mRNA levels and lower COL1A1 and VEGF levels than the SCFP-ASCs in 3D matrix. The difference in mRNA profile may have contributed to activation of the Akt, p38, RhoA, and JNK signaling pathways in the IPFP-ASCs. The chondrocytes differentiated from IPFP-ASCs had pronounced glycosaminoglycan and collagen type II production and a high chondroitin-6-sulfate/chondroitin-4-sulfate ratio with less polymerization of β-actin filaments. In an ex vivo mice model, magnetic resonance imaging revealed a shorter T2 relaxation time, indicating that more abundant extracellular matrix was secreted in the IPFP-ASC–matrix group. Histological examinations revealed that the IPFP-ASC matrix had higher chondrogenic efficacy of new cartilaginous tissue generation as evident in collagen type II and S-100 staining. Conclusion. ASCs isolated from IPFPs may be better candidates for cartilage regeneration, highlighting the translational potential of cartilage tissue engineering using the IPFP-ASC matrix technique.
Original language | English |
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Pages (from-to) | 508S-520S |
Journal | Cartilage |
Volume | 13 |
Issue number | 2_suppl |
DOIs | |
Publication status | Published - Jan 2021 |
Keywords
- adipose tissue–derived stem cells
- cartilage regeneration
- chondrogenic differentiation
- infrapatellar fat pad
- subcutaneous fat pad
ASJC Scopus subject areas
- Immunology and Allergy
- Biomedical Engineering
- Physical Therapy, Sports Therapy and Rehabilitation
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Dive into the research topics of 'Infrapatellar Fat Pads–Derived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An In Vitro and Ex Vivo Study Based on 3D Organized Self-Assembled Biomimetic Scaffold'. Together they form a unique fingerprint.Datasets
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sj-tif-2-car-10.1177_1947603520988153 – Supplemental material for Infrapatellar Fat Pads–Derived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An In Vitro and Ex Vivo Study Based on 3D Organized Self-Assembled Biomimetic Scaffold
Yang, Y. (Creator), Wang, C. (Contributor), Chen, Y. (Creator), Chen, I. (Creator) & Yang, K. (Creator), SAGE Journals, 2021
DOI: 10.25384/sage.13574128.v1, https://doi.org/10.25384%2Fsage.13574128.v1
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sj-pdf-3-car-10.1177_1947603520988153 – Supplemental material for Infrapatellar Fat Pads–Derived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An In Vitro and Ex Vivo Study Based on 3D Organized Self-Assembled Biomimetic Scaffold
Chen, Y. (Creator), Yang, K. (Creator), Yang, Y. (Creator), Wang, C. (Contributor) & Chen, I. (Creator), SAGE Journals, 2021
DOI: 10.25384/sage.13574122.v1, https://doi.org/10.25384%2Fsage.13574122.v1
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Infrapatellar Fat Pads–Derived Stem Cell Is a Favorable Cell Source for Articular Cartilage Tissue Engineering: An <i>In Vitro</i> and <i>Ex Vivo</i> Study Based on 3D Organized Self-Assembled Biomimetic Scaffold
Chen, Y. (Creator), Wang, C. (Contributor), Yang, K. (Creator), Yang, Y. (Creator) & Chen, I. (Creator), SAGE Journals, 2021
DOI: 10.25384/sage.c.5268931.v1, https://doi.org/10.25384%2Fsage.c.5268931.v1
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