Influenza A virus in Taiwan, 1980-2006

Phylogenetic and antigenic characteristics of the hemagglutinin gene

Sheng Fan Wang, Yuan Ming Lee, Yu Jiun Chan, Hsin Fu Liu, Yung Fong Yen, Wu Tse Liu, Jason C. Huang, Yi Ming Arthur Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Limited amount of information is available in Taiwan on the genetic or antigenic characteristics of influenza A virus prior to the establishment of a Taiwan surveillance network in 2000. Isolates of H1N1 and H3N2 viruses in Taiwan between 1980 and 2006 were studied, and part of the hemagglutinin gene was analyzed due to its importance in terms of viral infection and antibody neutralization. Results from a phylogenetic analysis indicate continuous evolutionary topology in H3N2 isolates, and two distinct H1N1 lineages. Many genetic relationships between vaccine strains and epidemic isolates appearing in Taiwan before other global locations were also observed and recorded in addition to a gradual increase in the number of N-linked glycosylation sites on partial HA1 proteins since 1980. The results from pairwise comparisons of HA1 nucleotide and deduced amino acid sequences indicate shared identities within groups organized according to their bootstrap and P-values of approximately 95.5-100% and 95.7-100% in H1N1 and 94.5-100% and 93.2-100% in H3N2 viruses, respectively. Comparisons of amino acid substitutions in the five antigenic regions reveal highly non-synonymous changes occurring in the Sb region of H1N1 and in the B region of H3N2. The results of an antigenic analysis using a hemagglutinin inhibition (HI) test indicate the presence of some epidemic strains 1-2 years earlier in Taiwan than in other parts of the world, as well as higher vaccine mismatch rates. This information supports the need for continuous surveillance of emerging influenza viruses in Taiwan, which will be useful for making global vaccine decisions.

Original languageEnglish
Pages (from-to)1457-1470
Number of pages14
JournalJournal of Medical Virology
Volume81
Issue number8
DOIs
Publication statusPublished - Aug 1 2009
Externally publishedYes

Fingerprint

Influenza A virus
Hemagglutinins
Taiwan
H3N2 Subtype Influenza A Virus
Genes
Vaccines
Viral Antibodies
H1N1 Subtype Influenza A Virus
Virus Diseases
Amino Acid Substitution
Orthomyxoviridae
Glycosylation
Amino Acid Sequence
Nucleotides
Proteins

Keywords

  • H1N1
  • H3N2
  • Hemagglutinin
  • Influenza
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Influenza A virus in Taiwan, 1980-2006 : Phylogenetic and antigenic characteristics of the hemagglutinin gene. / Wang, Sheng Fan; Lee, Yuan Ming; Chan, Yu Jiun; Liu, Hsin Fu; Yen, Yung Fong; Liu, Wu Tse; Huang, Jason C.; Chen, Yi Ming Arthur.

In: Journal of Medical Virology, Vol. 81, No. 8, 01.08.2009, p. 1457-1470.

Research output: Contribution to journalArticle

Wang, Sheng Fan ; Lee, Yuan Ming ; Chan, Yu Jiun ; Liu, Hsin Fu ; Yen, Yung Fong ; Liu, Wu Tse ; Huang, Jason C. ; Chen, Yi Ming Arthur. / Influenza A virus in Taiwan, 1980-2006 : Phylogenetic and antigenic characteristics of the hemagglutinin gene. In: Journal of Medical Virology. 2009 ; Vol. 81, No. 8. pp. 1457-1470.
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