Influence of hyperbaric oxygen on tumor necrosis factor-α and nitric oxide production in endotoxin-induced acute lung injury in rats

Shi Jye Chu, Min Hui Li, Ching Wang Hsu, Shih Hung Tsai, Shih Hua Lin, Kun Lun Huang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We previously demonstrated that hyperbaric oxygen (HBO) treatment alleviated lipopolysaccharide (LPS)-induced acute lung injury in rats. However, the mechanisms responsible for the protective effect are still not fully understood. To obtain further information on the protective effect of HBO, in this study we investigated the role of tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in intratracheal spraying LPS-induced acute lung injury in rats after HBO or hyperoxia treatment. The results showed that HBO but not hyperoxia attenuated the TNF-α level in plasma and bronchoalveolar lavage (BAL) fluid, NO concentration in BAL and plasma, and inducible NO synthase protein expression in lung tissue based on the Western blotting and immunohistochemical staining.

Original languageEnglish
Pages (from-to)684-690
Number of pages7
JournalPulmonary Pharmacology and Therapeutics
Volume20
Issue number6
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

Fingerprint

Acute Lung Injury
Endotoxins
Rats
Nitric Oxide
Tumor Necrosis Factor-alpha
Oxygen
Hyperoxia
Lipopolysaccharides
Plasmas
Bronchoalveolar Lavage Fluid
Bronchoalveolar Lavage
Nitric Oxide Synthase Type II
Spraying
Western Blotting
Tissue
Staining and Labeling
Lung
Fluids
Therapeutics
Proteins

Keywords

  • Acute lung injury
  • Hyperbaric oxygen
  • Nitric oxide
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pharmacology

Cite this

Influence of hyperbaric oxygen on tumor necrosis factor-α and nitric oxide production in endotoxin-induced acute lung injury in rats. / Chu, Shi Jye; Li, Min Hui; Hsu, Ching Wang; Tsai, Shih Hung; Lin, Shih Hua; Huang, Kun Lun.

In: Pulmonary Pharmacology and Therapeutics, Vol. 20, No. 6, 12.2007, p. 684-690.

Research output: Contribution to journalArticle

@article{9c0b145f64c842fda1aae973c2dda224,
title = "Influence of hyperbaric oxygen on tumor necrosis factor-α and nitric oxide production in endotoxin-induced acute lung injury in rats",
abstract = "We previously demonstrated that hyperbaric oxygen (HBO) treatment alleviated lipopolysaccharide (LPS)-induced acute lung injury in rats. However, the mechanisms responsible for the protective effect are still not fully understood. To obtain further information on the protective effect of HBO, in this study we investigated the role of tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in intratracheal spraying LPS-induced acute lung injury in rats after HBO or hyperoxia treatment. The results showed that HBO but not hyperoxia attenuated the TNF-α level in plasma and bronchoalveolar lavage (BAL) fluid, NO concentration in BAL and plasma, and inducible NO synthase protein expression in lung tissue based on the Western blotting and immunohistochemical staining.",
keywords = "Acute lung injury, Hyperbaric oxygen, Nitric oxide, Tumor necrosis factor-α",
author = "Chu, {Shi Jye} and Li, {Min Hui} and Hsu, {Ching Wang} and Tsai, {Shih Hung} and Lin, {Shih Hua} and Huang, {Kun Lun}",
year = "2007",
month = "12",
doi = "10.1016/j.pupt.2006.08.009",
language = "English",
volume = "20",
pages = "684--690",
journal = "Pulmonary Pharmacology and Therapeutics",
issn = "1094-5539",
publisher = "Academic Press Inc.",
number = "6",

}

TY - JOUR

T1 - Influence of hyperbaric oxygen on tumor necrosis factor-α and nitric oxide production in endotoxin-induced acute lung injury in rats

AU - Chu, Shi Jye

AU - Li, Min Hui

AU - Hsu, Ching Wang

AU - Tsai, Shih Hung

AU - Lin, Shih Hua

AU - Huang, Kun Lun

PY - 2007/12

Y1 - 2007/12

N2 - We previously demonstrated that hyperbaric oxygen (HBO) treatment alleviated lipopolysaccharide (LPS)-induced acute lung injury in rats. However, the mechanisms responsible for the protective effect are still not fully understood. To obtain further information on the protective effect of HBO, in this study we investigated the role of tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in intratracheal spraying LPS-induced acute lung injury in rats after HBO or hyperoxia treatment. The results showed that HBO but not hyperoxia attenuated the TNF-α level in plasma and bronchoalveolar lavage (BAL) fluid, NO concentration in BAL and plasma, and inducible NO synthase protein expression in lung tissue based on the Western blotting and immunohistochemical staining.

AB - We previously demonstrated that hyperbaric oxygen (HBO) treatment alleviated lipopolysaccharide (LPS)-induced acute lung injury in rats. However, the mechanisms responsible for the protective effect are still not fully understood. To obtain further information on the protective effect of HBO, in this study we investigated the role of tumor necrosis factor-α (TNF-α) and nitric oxide (NO) in intratracheal spraying LPS-induced acute lung injury in rats after HBO or hyperoxia treatment. The results showed that HBO but not hyperoxia attenuated the TNF-α level in plasma and bronchoalveolar lavage (BAL) fluid, NO concentration in BAL and plasma, and inducible NO synthase protein expression in lung tissue based on the Western blotting and immunohistochemical staining.

KW - Acute lung injury

KW - Hyperbaric oxygen

KW - Nitric oxide

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=34548827340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548827340&partnerID=8YFLogxK

U2 - 10.1016/j.pupt.2006.08.009

DO - 10.1016/j.pupt.2006.08.009

M3 - Article

VL - 20

SP - 684

EP - 690

JO - Pulmonary Pharmacology and Therapeutics

JF - Pulmonary Pharmacology and Therapeutics

SN - 1094-5539

IS - 6

ER -