Abstract

Platelet gels (PG) are new topical single-donor blood products which are attracting great interest in regenerative medicine. They are obtained by mixing a platelet-rich plasma fraction with thrombin to generate a fibrin gel enriched in platelet growth factors (GF). The type of thrombin preparation may affect PG reproducibility. We have determined the impact of 14.6% (v/v) ethanol-stabilized thrombin (EHT) on the release of GF by platelets. Various ratios of EHT and platelet concentrates were mixed to obtain from 2.43 to 7.96% ethanol concentration. Platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-ß1 (TGF-ß1), vascular endothelium growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were assessed at 5, 120, and 300 min after PG formation. Protein profiles of thrombin and PG releasates were analyzed by SDS-PAGE. The amount of PDGF-AB, TGF-ß1, and VEGF released per platelet decreased significantly (p <0.05) with increasing ethanol concentrations but, however, not that of EGF. IGF-1 content was stable, consistent with its presence mostly in plasma. SDS-PAGE indicated that ethanol did not affect fibrin formation. In conclusion, ethanol has a significant impact on the amount of GF released by platelets and should be strictly controlled to standardize PG and optimize clinical benefits.

Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalBiologicals
Volume38
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Platelets
Intercellular Signaling Peptides and Proteins
Blood
Ethanol
Blood Platelets
Gels
Thrombin
Vascular Endothelium
Transforming Growth Factors
Somatomedins
Fibrin
Epidermal Growth Factor
Insulin
Polyacrylamide Gel Electrophoresis
Platelet-Rich Plasma
Plasmas
Regenerative Medicine
Blood Donors
Proteins

Keywords

  • EGF
  • Ethanol
  • Growth factors
  • IGF-1
  • PDGF-AB
  • Platelet gel
  • TGF-β
  • Thrombin
  • VEGF

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Immunology and Microbiology(all)
  • Pharmacology

Cite this

Influence of ethanol on the release of growth factors in human blood-derived platelet gels. / Kuo, Ya Po; Lee, Yueh Lun; Tseng, Yu Hong; Su, Ching Hua; Burnouf, Thierry; Su, Chen Yao.

In: Biologicals, Vol. 38, No. 1, 01.2010, p. 120-127.

Research output: Contribution to journalArticle

Kuo, Ya Po ; Lee, Yueh Lun ; Tseng, Yu Hong ; Su, Ching Hua ; Burnouf, Thierry ; Su, Chen Yao. / Influence of ethanol on the release of growth factors in human blood-derived platelet gels. In: Biologicals. 2010 ; Vol. 38, No. 1. pp. 120-127.
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abstract = "Platelet gels (PG) are new topical single-donor blood products which are attracting great interest in regenerative medicine. They are obtained by mixing a platelet-rich plasma fraction with thrombin to generate a fibrin gel enriched in platelet growth factors (GF). The type of thrombin preparation may affect PG reproducibility. We have determined the impact of 14.6{\%} (v/v) ethanol-stabilized thrombin (EHT) on the release of GF by platelets. Various ratios of EHT and platelet concentrates were mixed to obtain from 2.43 to 7.96{\%} ethanol concentration. Platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-{\ss}1 (TGF-{\ss}1), vascular endothelium growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were assessed at 5, 120, and 300 min after PG formation. Protein profiles of thrombin and PG releasates were analyzed by SDS-PAGE. The amount of PDGF-AB, TGF-{\ss}1, and VEGF released per platelet decreased significantly (p <0.05) with increasing ethanol concentrations but, however, not that of EGF. IGF-1 content was stable, consistent with its presence mostly in plasma. SDS-PAGE indicated that ethanol did not affect fibrin formation. In conclusion, ethanol has a significant impact on the amount of GF released by platelets and should be strictly controlled to standardize PG and optimize clinical benefits.",
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AB - Platelet gels (PG) are new topical single-donor blood products which are attracting great interest in regenerative medicine. They are obtained by mixing a platelet-rich plasma fraction with thrombin to generate a fibrin gel enriched in platelet growth factors (GF). The type of thrombin preparation may affect PG reproducibility. We have determined the impact of 14.6% (v/v) ethanol-stabilized thrombin (EHT) on the release of GF by platelets. Various ratios of EHT and platelet concentrates were mixed to obtain from 2.43 to 7.96% ethanol concentration. Platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-ß1 (TGF-ß1), vascular endothelium growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were assessed at 5, 120, and 300 min after PG formation. Protein profiles of thrombin and PG releasates were analyzed by SDS-PAGE. The amount of PDGF-AB, TGF-ß1, and VEGF released per platelet decreased significantly (p <0.05) with increasing ethanol concentrations but, however, not that of EGF. IGF-1 content was stable, consistent with its presence mostly in plasma. SDS-PAGE indicated that ethanol did not affect fibrin formation. In conclusion, ethanol has a significant impact on the amount of GF released by platelets and should be strictly controlled to standardize PG and optimize clinical benefits.

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