Induction of VX2 carcinoma in rabbit liver

Comparison of two inoculation methods

J. H. Chen, Y. C. Lin, Y. S. Huang, T. J. Chen, W. Y. Lin, K. W. Han

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Direct injection of VX2 cell suspension into the liver is simple and widely used. Implantation of a fragment of VX2 tumour into the liver using a surgical technique has also been developed in the last decade. In this study, we compared these two methods in order to find a better modality for establishing VX2 liver mass. Forty rabbits, each weighing 2.8-3.2 kg, were divided into two groups, 20 rabbits in each. In Group 1, a tumour cell suspension containing 1 × 106 cells in a volume of 0.1 ml, was injected slowly into the liver parenchyma using a 27-gauge needle during laparotomy. In Group 2, a 1 mm3 fragment of VX2 carcinoma was inoculated into the sub-capsule of the left anterior lobe of the liver. In Group 1, three rabbits showed no tumour growth and 10 rabbits showed evidence of leakage and tumour seeding outside of the liver. In Group 2, all but one rabbit showed tumour growth and none showed evidence of tumour seeding. The leakage rates were 50% and 0% for Group 1 and Group 2, respectively. Overall, the success inoculation rate was 35% for Group 1 and 95% for Group 2. In conclusion, to create the VX2 liver tumour model in rabbits, direct implantation of VX2 tumour fragment into the liver achieved better results than injecting cell suspension of VX2 tumour into the liver.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalLaboratory Animals
Volume38
Issue number1
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

Fingerprint

inoculation methods
carcinoma
rabbits
Rabbits
Carcinoma
liver
liver neoplasms
Liver
neoplasms
cell suspension culture
Neoplasms
Suspensions
sowing
laparotomy
gauges
surgery
Growth
injection
Laparotomy
Capsules

Keywords

  • Carcinoma
  • Cell
  • Implantation
  • Injection
  • Liver
  • Rabbit
  • Tumour

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Induction of VX2 carcinoma in rabbit liver : Comparison of two inoculation methods. / Chen, J. H.; Lin, Y. C.; Huang, Y. S.; Chen, T. J.; Lin, W. Y.; Han, K. W.

In: Laboratory Animals, Vol. 38, No. 1, 01.2004, p. 79-84.

Research output: Contribution to journalArticle

Chen, J. H. ; Lin, Y. C. ; Huang, Y. S. ; Chen, T. J. ; Lin, W. Y. ; Han, K. W. / Induction of VX2 carcinoma in rabbit liver : Comparison of two inoculation methods. In: Laboratory Animals. 2004 ; Vol. 38, No. 1. pp. 79-84.
@article{c3c8e5532cb848e58ae63eb91dc0da11,
title = "Induction of VX2 carcinoma in rabbit liver: Comparison of two inoculation methods",
abstract = "Direct injection of VX2 cell suspension into the liver is simple and widely used. Implantation of a fragment of VX2 tumour into the liver using a surgical technique has also been developed in the last decade. In this study, we compared these two methods in order to find a better modality for establishing VX2 liver mass. Forty rabbits, each weighing 2.8-3.2 kg, were divided into two groups, 20 rabbits in each. In Group 1, a tumour cell suspension containing 1 × 106 cells in a volume of 0.1 ml, was injected slowly into the liver parenchyma using a 27-gauge needle during laparotomy. In Group 2, a 1 mm3 fragment of VX2 carcinoma was inoculated into the sub-capsule of the left anterior lobe of the liver. In Group 1, three rabbits showed no tumour growth and 10 rabbits showed evidence of leakage and tumour seeding outside of the liver. In Group 2, all but one rabbit showed tumour growth and none showed evidence of tumour seeding. The leakage rates were 50{\%} and 0{\%} for Group 1 and Group 2, respectively. Overall, the success inoculation rate was 35{\%} for Group 1 and 95{\%} for Group 2. In conclusion, to create the VX2 liver tumour model in rabbits, direct implantation of VX2 tumour fragment into the liver achieved better results than injecting cell suspension of VX2 tumour into the liver.",
keywords = "Carcinoma, Cell, Implantation, Injection, Liver, Rabbit, Tumour",
author = "Chen, {J. H.} and Lin, {Y. C.} and Huang, {Y. S.} and Chen, {T. J.} and Lin, {W. Y.} and Han, {K. W.}",
year = "2004",
month = "1",
doi = "10.1258/00236770460734434",
language = "English",
volume = "38",
pages = "79--84",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "SAGE Publications Ltd",
number = "1",

}

TY - JOUR

T1 - Induction of VX2 carcinoma in rabbit liver

T2 - Comparison of two inoculation methods

AU - Chen, J. H.

AU - Lin, Y. C.

AU - Huang, Y. S.

AU - Chen, T. J.

AU - Lin, W. Y.

AU - Han, K. W.

PY - 2004/1

Y1 - 2004/1

N2 - Direct injection of VX2 cell suspension into the liver is simple and widely used. Implantation of a fragment of VX2 tumour into the liver using a surgical technique has also been developed in the last decade. In this study, we compared these two methods in order to find a better modality for establishing VX2 liver mass. Forty rabbits, each weighing 2.8-3.2 kg, were divided into two groups, 20 rabbits in each. In Group 1, a tumour cell suspension containing 1 × 106 cells in a volume of 0.1 ml, was injected slowly into the liver parenchyma using a 27-gauge needle during laparotomy. In Group 2, a 1 mm3 fragment of VX2 carcinoma was inoculated into the sub-capsule of the left anterior lobe of the liver. In Group 1, three rabbits showed no tumour growth and 10 rabbits showed evidence of leakage and tumour seeding outside of the liver. In Group 2, all but one rabbit showed tumour growth and none showed evidence of tumour seeding. The leakage rates were 50% and 0% for Group 1 and Group 2, respectively. Overall, the success inoculation rate was 35% for Group 1 and 95% for Group 2. In conclusion, to create the VX2 liver tumour model in rabbits, direct implantation of VX2 tumour fragment into the liver achieved better results than injecting cell suspension of VX2 tumour into the liver.

AB - Direct injection of VX2 cell suspension into the liver is simple and widely used. Implantation of a fragment of VX2 tumour into the liver using a surgical technique has also been developed in the last decade. In this study, we compared these two methods in order to find a better modality for establishing VX2 liver mass. Forty rabbits, each weighing 2.8-3.2 kg, were divided into two groups, 20 rabbits in each. In Group 1, a tumour cell suspension containing 1 × 106 cells in a volume of 0.1 ml, was injected slowly into the liver parenchyma using a 27-gauge needle during laparotomy. In Group 2, a 1 mm3 fragment of VX2 carcinoma was inoculated into the sub-capsule of the left anterior lobe of the liver. In Group 1, three rabbits showed no tumour growth and 10 rabbits showed evidence of leakage and tumour seeding outside of the liver. In Group 2, all but one rabbit showed tumour growth and none showed evidence of tumour seeding. The leakage rates were 50% and 0% for Group 1 and Group 2, respectively. Overall, the success inoculation rate was 35% for Group 1 and 95% for Group 2. In conclusion, to create the VX2 liver tumour model in rabbits, direct implantation of VX2 tumour fragment into the liver achieved better results than injecting cell suspension of VX2 tumour into the liver.

KW - Carcinoma

KW - Cell

KW - Implantation

KW - Injection

KW - Liver

KW - Rabbit

KW - Tumour

UR - http://www.scopus.com/inward/record.url?scp=1642422240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642422240&partnerID=8YFLogxK

U2 - 10.1258/00236770460734434

DO - 10.1258/00236770460734434

M3 - Article

VL - 38

SP - 79

EP - 84

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

IS - 1

ER -