Induction of secretory phospholipase A2 in reactive astrocytes in response to transient focal cerebral ischemia in the rat brain

Teng Nan Lin, Qun Wang, Agnes Simonyi, Jean Ju Chen, Wai Mui Cheung, Yong Y. He, Jan Xu, Albert Y. Sun, Chung Y. Hsu, Grace Y. Sun

Research output: Contribution to journalArticle

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Abstract

Although mRNA expression of group IIA secretory phospholipase A2 (sPLA2-IIA) has been implicated in responses to injury in the CNS, information on protein expression remains unclear. In this study, we investigated temporal and spatial expression of sPLA2-IIA mRNA and immunoreactivity in transient focal cerebral ischemia induced in rats by occlusion of the middle cerebral artery. Northern blot analysis showed a biphasic increase in sPLA2-IIA mRNA expression following 60-min of ischemia-reperfusion: an early phase at 30 min and a second increase at a late phase ranging from 12 h to 14 days. In situ hybridization localized the early-phase increase in sPLA2-IIA mRNA to the affected ischemic cortex and the late-phase increase to the penumbral area. Besides sPLA 2-IIA mRNA, glial fibrillary acidic protein (GFAP) and cyclo-oxygenase-2 mRNAs, but not cytosolic PLA2, also showed an increase in the penumbral area at 3 days after ischemia-reperfusion. Immunohistochemistry of sPLA2-IIA indicated positive cells in the penumbral area similar to the GFAP-positive astrocytes but different from the isolectin B4-positive microglial cells. Confocal microscopy further confirmed immunoreactivity of sPLA2-IIA in reactive astrocytes but not in microglial cells. Taken together, these results demonstrate for the first time an up-regulation of the inflammatory sPLA2-IIA in reactive astrocytes in response to cerebral ischemia-reperfusion.

Original languageEnglish
Pages (from-to)637-645
Number of pages9
JournalJournal of Neurochemistry
Volume90
Issue number3
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Secretory Phospholipase A2
Transient Ischemic Attack
Astrocytes
Rats
Brain
Messenger RNA
Reperfusion
Glial Fibrillary Acidic Protein
Ischemia
Group II Phospholipases A2
Confocal microscopy
Middle Cerebral Artery Infarction
Prostaglandin-Endoperoxide Synthases
Brain Ischemia
Lectins
Confocal Microscopy
Northern Blotting
In Situ Hybridization
Up-Regulation
Immunohistochemistry

Keywords

  • Astrocytes
  • Cerebral ischemia
  • Group IIA secretory phospholipase A
  • Immunohistochemistry
  • In situ hybridization
  • Microglial cells

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Induction of secretory phospholipase A2 in reactive astrocytes in response to transient focal cerebral ischemia in the rat brain. / Lin, Teng Nan; Wang, Qun; Simonyi, Agnes; Chen, Jean Ju; Cheung, Wai Mui; He, Yong Y.; Xu, Jan; Sun, Albert Y.; Hsu, Chung Y.; Sun, Grace Y.

In: Journal of Neurochemistry, Vol. 90, No. 3, 08.2004, p. 637-645.

Research output: Contribution to journalArticle

Lin, TN, Wang, Q, Simonyi, A, Chen, JJ, Cheung, WM, He, YY, Xu, J, Sun, AY, Hsu, CY & Sun, GY 2004, 'Induction of secretory phospholipase A2 in reactive astrocytes in response to transient focal cerebral ischemia in the rat brain', Journal of Neurochemistry, vol. 90, no. 3, pp. 637-645. https://doi.org/10.1111/j.1471-4159.2004.02540.x
Lin, Teng Nan ; Wang, Qun ; Simonyi, Agnes ; Chen, Jean Ju ; Cheung, Wai Mui ; He, Yong Y. ; Xu, Jan ; Sun, Albert Y. ; Hsu, Chung Y. ; Sun, Grace Y. / Induction of secretory phospholipase A2 in reactive astrocytes in response to transient focal cerebral ischemia in the rat brain. In: Journal of Neurochemistry. 2004 ; Vol. 90, No. 3. pp. 637-645.
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AU - Cheung, Wai Mui

AU - He, Yong Y.

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AU - Sun, Albert Y.

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