Induction of pulmonary allergic responses by antigen-specific Th2 cells

Xiu Min Li, Brian H. Schofield, Qian Fei Wang, Kawn Hyoung Kim, Shau Ku Huang

Research output: Contribution to journalArticle

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Abstract

The development of pulmonary allergic responses was examined in mice following pulmonary transfer of Ag (conalbumin)-specific Th2 cells. The levels of serum-specific IgE, cellular infiltrates, airway mucus goblet cells, and airway responsiveness were analyzed and compared with those in Ag- sensitized and -challenged mice. Pulmonary transfer of the conalbumin- specific Th2 clone (D10) induced, in an Ag-specific manner, high levels of the Th2 cytokines IL-4 and IL-5 in the bronchoalveolar lavage fluids and mucosal eosinophils, concomitant with an increase in airway responsiveness. The D10 cell-induced responses were seen in the absence of serum specific IgE. In the presence of Ag, the transferred D10 cells not only remained in the lungs, but also increased in number 72 h post-cell transfer. Although significantly higher levels of IL-4 and IL-5 in the bronchoalveolar lavage fluids were found in D10-transferred mice, the levels of pulmonary eosinophilia, mucus goblet cells, and airway responsiveness were significantly lower than those in Ag-sensitized and -challenged mice. These results demonstrate that although Ag-specific activation of Th2 cells at mucosal sites is able to mediate the recruitment of eosinophils and the subsequent induction of airway hyper-responsiveness, the more severe pulmonary allergic responses were observed only in mice sensitized and challenged with Ag.

Original languageEnglish
Pages (from-to)1378-1384
Number of pages7
JournalJournal of Immunology
Volume160
Issue number3
Publication statusPublished - Feb 1 1998
Externally publishedYes

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Th2 Cells
Antigens
Lung
Conalbumin
Goblet Cells
Interleukin-5
Bronchoalveolar Lavage Fluid
Mucus
Eosinophils
Interleukin-4
Immunoglobulin E
Respiratory Hypersensitivity
Pulmonary Eosinophilia
Serum
Clone Cells
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Li, X. M., Schofield, B. H., Wang, Q. F., Kim, K. H., & Huang, S. K. (1998). Induction of pulmonary allergic responses by antigen-specific Th2 cells. Journal of Immunology, 160(3), 1378-1384.

Induction of pulmonary allergic responses by antigen-specific Th2 cells. / Li, Xiu Min; Schofield, Brian H.; Wang, Qian Fei; Kim, Kawn Hyoung; Huang, Shau Ku.

In: Journal of Immunology, Vol. 160, No. 3, 01.02.1998, p. 1378-1384.

Research output: Contribution to journalArticle

Li, XM, Schofield, BH, Wang, QF, Kim, KH & Huang, SK 1998, 'Induction of pulmonary allergic responses by antigen-specific Th2 cells', Journal of Immunology, vol. 160, no. 3, pp. 1378-1384.
Li XM, Schofield BH, Wang QF, Kim KH, Huang SK. Induction of pulmonary allergic responses by antigen-specific Th2 cells. Journal of Immunology. 1998 Feb 1;160(3):1378-1384.
Li, Xiu Min ; Schofield, Brian H. ; Wang, Qian Fei ; Kim, Kawn Hyoung ; Huang, Shau Ku. / Induction of pulmonary allergic responses by antigen-specific Th2 cells. In: Journal of Immunology. 1998 ; Vol. 160, No. 3. pp. 1378-1384.
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