Induction of phenolsulfotransferase expression by phenolic acids in human hepatoma HepG2 cells

Chi Tai Yeh, Shang Ming Huang, Gow Chin Yen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Phenolic acids are antioxidant phenolic compounds, widespread in plant foods, which contribute significant biological and pharmacological properties; some have demonstrated a remarkable ability to alter sulfate conjugation. However, the modulation mechanisms of antioxidant phenolic acids on phenolsulfotransferase activity have not yet been described. In the present study, the human hepatoma cell line, HepG2, was used as a model to investigate the effect of antioxidant phenolic acids on enzymatic activity and expression of one of the major phase II sulfate conjugation enzymes, P-form phenolsulfotransferase (PST-P). The results showed that gallic acid, gentisic acid, p-hydroxybenzoic acid, and p-coumaric acid increased PST-P activity, in a dose-dependent manner. A maximum of 4-and 5-fold induction of PST-P activity was observed for both gallic acid and gentisic acid; however, they showed an adverse effect on cell growth at higher concentrations. A 2- or 2.5-fold increase of PST-P activity was found with either p-coumaric or p-hydroxybenzoic acid treatment, whereas no significant effect was found for ferulic acid treatment. PST-P induction, by gallic acid, was further confirmed, using reverse transcription PCR and Western blotting techniques to measure mRNA expression and protein translation. A significant correlation (r = 0.74, p < 0.01) between the expressions of PST-P mRNA and the corresponding PST-P activity was observed. Thus, gallic acid increased PST-P protein expression in HepG2 cells, in a dose- and time-dependent manner. The results demonstrated that certain antioxidant phenolic acids could induce PST-P activity in HepG2 cells, by promoting PST-P mRNA and protein expression, suggesting a novel mechanism by which phenolic acids may be implicated in phase II sulfate conjugation.

Original languageEnglish
Pages (from-to)4766-4773
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number12
DOIs
Publication statusPublished - Jun 15 2005
Externally publishedYes

Fingerprint

Hep G2 Cells
hepatoma
phenolic acids
Gallic Acid
Hepatocellular Carcinoma
gallic acid
Antioxidants
2,5-dihydroxybenzoic acid
Sulfates
Hydroxybenzoates
antioxidants
sulfates
4-hydroxybenzoic acid
ferulic acid
Protein Biosynthesis
acid treatment
Messenger RNA
cells
Arylsulfotransferase
protein synthesis

Keywords

  • Human hepatoma cell line HepG
  • P-form phenolsulfotransferase
  • Phenolic acids
  • Sulfation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Induction of phenolsulfotransferase expression by phenolic acids in human hepatoma HepG2 cells. / Yeh, Chi Tai; Huang, Shang Ming; Yen, Gow Chin.

In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 12, 15.06.2005, p. 4766-4773.

Research output: Contribution to journalArticle

Yeh, Chi Tai ; Huang, Shang Ming ; Yen, Gow Chin. / Induction of phenolsulfotransferase expression by phenolic acids in human hepatoma HepG2 cells. In: Journal of Agricultural and Food Chemistry. 2005 ; Vol. 53, No. 12. pp. 4766-4773.
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abstract = "Phenolic acids are antioxidant phenolic compounds, widespread in plant foods, which contribute significant biological and pharmacological properties; some have demonstrated a remarkable ability to alter sulfate conjugation. However, the modulation mechanisms of antioxidant phenolic acids on phenolsulfotransferase activity have not yet been described. In the present study, the human hepatoma cell line, HepG2, was used as a model to investigate the effect of antioxidant phenolic acids on enzymatic activity and expression of one of the major phase II sulfate conjugation enzymes, P-form phenolsulfotransferase (PST-P). The results showed that gallic acid, gentisic acid, p-hydroxybenzoic acid, and p-coumaric acid increased PST-P activity, in a dose-dependent manner. A maximum of 4-and 5-fold induction of PST-P activity was observed for both gallic acid and gentisic acid; however, they showed an adverse effect on cell growth at higher concentrations. A 2- or 2.5-fold increase of PST-P activity was found with either p-coumaric or p-hydroxybenzoic acid treatment, whereas no significant effect was found for ferulic acid treatment. PST-P induction, by gallic acid, was further confirmed, using reverse transcription PCR and Western blotting techniques to measure mRNA expression and protein translation. A significant correlation (r = 0.74, p < 0.01) between the expressions of PST-P mRNA and the corresponding PST-P activity was observed. Thus, gallic acid increased PST-P protein expression in HepG2 cells, in a dose- and time-dependent manner. The results demonstrated that certain antioxidant phenolic acids could induce PST-P activity in HepG2 cells, by promoting PST-P mRNA and protein expression, suggesting a novel mechanism by which phenolic acids may be implicated in phase II sulfate conjugation.",
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