Induction of nephrotoxic serum nephritis in inbred mice and suppressive effect of colchicine on the development of this nephritis

Shih Ming Chen, Takuya Mukoyama, Noriko Sato, Shin Ichi Yamagata, Yuichiro Arai, Nobunori Satoh, Shiro Ueda

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10 Citations (Scopus)

Abstract

Accelerated nephrotoxic serum (NTS) nephritis is successfully produced in C57BL/6 mice, using anti-murine glomerular basement membrane (GBM) rabbit antiserum. Anti-murine GBM rabbits antiserum was obtained by immunization of New Zealand white rabbit with trypsinized GBM antigen from normal C57BL/6 mice. Preimmunization with normal rabbit IgG and injection with 150 μl of NTS induced typical NTS nephritis with cellular proliferation in glomeruli, occlusion of glomerular loops, crescents, tubulointerstitial changes and hyperazotemia within 14 days. Polymorphonuclear leucocytes (PMN) have an important role in induction and development of NTS nephritis. Furthermore, clinically used colchicine is thought to suppress functions of PMN. Therefore, the therapeutic effect of colchicine on NTS nephritis was examined. The histological score (HS) of the group treated with 60 μg kg-1 of colchicine (2.8 ± 0.5) was significantly lower than that of positive control group (4.03 ± 0.3). The direct immunofluorescent microscopic study revealed that there is no quantitative difference in the deposition of rabbit IgG, mouse IgG and C3 in GBM between these two groups. Urinary protein excretion and hyperazotemia were significantly suppressed by treatment with 60 μg kg(-)1 of colchicine. A NTS nephritis model was established, it was found that colchicine may have a suppressive effect on the development of glomerular nephritis.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalPharmacological Research
Volume45
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

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Keywords

  • Anti-glomerular basement membrane antibody (anti-GBM antibody)
  • Colchicine
  • Mouse
  • Nephrotoxic serum nephritis (NTS nephritis)
  • Polymorphonuclear leucocytes (PMN)

ASJC Scopus subject areas

  • Pharmacology

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