Induction of heme oxygenase-1 expression in vascular smooth muscle cells. A link to endotoxic shock

Shaw Fang Yet, Andrea Pellacani, Cam Patterson, Larissa Tan, Sara C. Folta, Lauren Foster, Wen Sen Lee, Chung Ming Hsieh, Mark A. Perrella

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Endotoxic shock is a life-threatening consequence of severe Gram- negative infection characterized by vascular smooth muscle cell relaxation and severe hypotension. The production of nitric oxide (NO), through the inducible NO synthase pathway, has been implicated as a major contributor in this process. We now demonstrate that heme oxygenase (HO), an enzyme that generates carbon monoxide (CO) in the course of heme metabolism, may also be involved in the hemodynamic compromise of endotoxic shock. Inducible HO (HO- 1) mRNA levels are dramatically increased in aortic tissue from rats receiving endotoxin, and this increase in vascular HO-1 message is associated with an 8.9-fold increase in HO enzyme activity in vivo. Immunocytochemical staining localizes an increase in HO-1 protein within smooth muscle cells of both large (aorta) and small (arterioles) blood vessels. Furthermore, zinc protoporphyrin IX, an inhibitor of HO activity, abrogates endotoxin-induced hypotension in rats. Studies performed in rat vascular smooth muscle cells in vitro show that the induction of HO-1 mRNA is regulated at the level of gene transcription, and this induction is independent of NO production. Taken together, these studies suggest that the up-regulation of HO-1, and the subsequent production of CO, contributes to the reduction in vascular tone during endotoxic shock.

Original languageEnglish
Pages (from-to)4295-4301
Number of pages7
JournalJournal of Biological Chemistry
Volume272
Issue number7
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Heme Oxygenase-1
Heme Oxygenase (Decyclizing)
Septic Shock
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Cells
Blood Vessels
Rats
Carbon Monoxide
Endotoxins
Nitric Oxide
Controlled Hypotension
Messenger RNA
Muscle Relaxation
Blood vessels
Hemodynamics
Enzyme activity
Arterioles
Nitric Oxide Synthase Type II

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yet, S. F., Pellacani, A., Patterson, C., Tan, L., Folta, S. C., Foster, L., ... Perrella, M. A. (1997). Induction of heme oxygenase-1 expression in vascular smooth muscle cells. A link to endotoxic shock. Journal of Biological Chemistry, 272(7), 4295-4301. https://doi.org/10.1074/jbc.272.7.4295

Induction of heme oxygenase-1 expression in vascular smooth muscle cells. A link to endotoxic shock. / Yet, Shaw Fang; Pellacani, Andrea; Patterson, Cam; Tan, Larissa; Folta, Sara C.; Foster, Lauren; Lee, Wen Sen; Hsieh, Chung Ming; Perrella, Mark A.

In: Journal of Biological Chemistry, Vol. 272, No. 7, 1997, p. 4295-4301.

Research output: Contribution to journalArticle

Yet, SF, Pellacani, A, Patterson, C, Tan, L, Folta, SC, Foster, L, Lee, WS, Hsieh, CM & Perrella, MA 1997, 'Induction of heme oxygenase-1 expression in vascular smooth muscle cells. A link to endotoxic shock', Journal of Biological Chemistry, vol. 272, no. 7, pp. 4295-4301. https://doi.org/10.1074/jbc.272.7.4295
Yet, Shaw Fang ; Pellacani, Andrea ; Patterson, Cam ; Tan, Larissa ; Folta, Sara C. ; Foster, Lauren ; Lee, Wen Sen ; Hsieh, Chung Ming ; Perrella, Mark A. / Induction of heme oxygenase-1 expression in vascular smooth muscle cells. A link to endotoxic shock. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 7. pp. 4295-4301.
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