Induction of Hemagglutination by Recombinant Porphyromonas Gingivalis 40-kDa Outer Membrane Protein

Yoshimitsu Abiko, Koichi Hiratsuka, Michiko Kiyama-Kishikawa, Mitsuo Hayakawa, Hiroaki Tagawa, Mau-Chang Lin, Wei-Jen Chang, Sheng-Yang Lee

Research output: Contribution to journalArticle

Abstract

Porphyromonas gingivalis, an important pathogen in periodontitis, possesses strong hemagglutinating activity. This is mediated by a variety of hemagglutinating molecules on its cell surface. We previously succeeded in a cloning the gene encoding 40-kDa outer membrane protein (40-kDa OMP) from P. gingivalis 381. To clarify the pathological role of 40-kDa OMP, we examined its hemagglutinating activity. The recombinant 40-kDa OMP (r40-kDa OMP) was highly purified and used to raise a rabbit antiserum, which was then purified by r40-kDa OMP affinity chromatography. Although the r40-kDa OMP itself did not show hemagglutinating activity, a cross-linked polymeric form had strong activity. Finally, the affinity-purified anti-r40-kDa OMP antibody inhibited hemagglutination caused by P. gingivalis vesicles. These findings suggest that 40-kDa OMP is a novel hemagglutinin that plays an important role in the pathogenicity of P. gingivalis.
Original languageEnglish
Pages (from-to)54-56
Number of pages3
JournalInternational Journal of Oral-Medical Sciences
Volume2
Issue number1
DOIs
Publication statusPublished - 2004

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