Induction of fatty acid cyclooxygenase in rat aortic smooth muscle cells by estradiol

Wen Chang Chang; Junko Nakao; Sei itsu Murota; Hsin Hsiung Tai

doi:10.1016/S0262-1746(83)80018-3

Induction of fatty acid cyclooxygenase in rat aortic smooth muscle cells by estradiol

Wen Chang Chang, Junko Nakao, Sei itsu Murota, Hsin Hsiung Tai

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.

Original language	English
Pages (from-to)	33-37
Number of pages	5
Journal	Prostaglandins, Leukotrienes and Medicine
Volume	10
Issue number	1
DOIs	https://doi.org/10.1016/S0262-1746(83)80018-3
Publication status	Published - Jan 1983
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology

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title = "Induction of fatty acid cyclooxygenase in rat aortic smooth muscle cells by estradiol",

abstract = "We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.",

author = "Chang, {Wen Chang} and Junko Nakao and Murota, {Sei itsu} and Tai, {Hsin Hsiung}",

note = "Funding Information: This work was supported in part from the National Institutes of Health (GM-30380), the American Heart Association (81-1152), the Kentucky Heart Association and the University of Kentucky Isabell L, Kircher Fund.",

year = "1983",

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AU - Chang, Wen Chang

AU - Nakao, Junko

AU - Murota, Sei itsu

AU - Tai, Hsin Hsiung

N1 - Funding Information: This work was supported in part from the National Institutes of Health (GM-30380), the American Heart Association (81-1152), the Kentucky Heart Association and the University of Kentucky Isabell L, Kircher Fund.

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N2 - We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.

AB - We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.

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