Abstract
We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.
| Original language | English |
|---|---|
| Pages (from-to) | 33-37 |
| Number of pages | 5 |
| Journal | Prostaglandins, Leukotrienes and Medicine |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
Cite this
Induction of fatty acid cyclooxygenase in rat aortic smooth muscle cells by estradiol. / Chang, Wen Chang; Nakao, Junko; Murota, Sei itsu; Tai, Hsin Hsiung.
In: Prostaglandins, Leukotrienes and Medicine, Vol. 10, No. 1, 1983, p. 33-37.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of fatty acid cyclooxygenase in rat aortic smooth muscle cells by estradiol
AU - Chang, Wen Chang
AU - Nakao, Junko
AU - Murota, Sei itsu
AU - Tai, Hsin Hsiung
PY - 1983
Y1 - 1983
N2 - We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.
AB - We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.
UR - http://www.scopus.com/inward/record.url?scp=0020693279&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020693279&partnerID=8YFLogxK
U2 - 10.1016/S0262-1746(83)80018-3
DO - 10.1016/S0262-1746(83)80018-3
M3 - Article
C2 - 6402792
AN - SCOPUS:0020693279
VL - 10
SP - 33
EP - 37
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
IS - 1
ER -