Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells

Bin Chuan Ji, Chien Chih Yu, S. U Tso Yang, T. E Chun Hsia, Jai Sing Yang, Kuang Chi Lai, Yang Ching Ko, Jen Jyh Lin, Tung-Iuan Lai, Jing Gung Chung

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

It has been shown that deguelin, one of the compounds of rotenoids from flavonoid family, induced cytotoxic effects through induction of cell cycle arrest and apoptosis in many types of human cancer cell lines, but deguelin-affected DNA damage and repair gene expression (mRNA) are not clarified yet. We investigated the effects of deguelin on DNA damage and associated gene expression in human lung cancer NCI-H460 cells in vitro. DNA damage was assayed by using the comet assay and DNA gel electrophoresis and the results indicated that NCI-H460 cells treated with 0, 50, 250 and 500 nM deguelin led to a longer DNA migration smear based on the single cell electrophoresis and DNA fragmentation occurred based on the examination of DNA gel electrophoresis. DNA damage and repair gene expression (mRNA) were evaluated by using real-time PCR assay and the results indicated that 50 and 250 nM deguelin for a 24-h exposure in NCI-H460 cells, decreased the gene levels of breast cancer 1, early onset (BRCA1), DNA-dependent serine/threonine protein kinase (DNA-PK), O 6-methylguanine-DNA methyltransferase (MGMT), p53, ataxia telangiectasia mutated (ATM) and ataxia-telangiectasia and Rad3-related (ATR) mRNA expressions. Collectively, the present study showed that deguelin caused DNA damage and inhibited DNA damage and repair gene expressions, which might be due to deguelin-inhibited cell growth in vitro.

Original languageEnglish
Pages (from-to)959-964
Number of pages6
JournalOncology Reports
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 2012

Keywords

  • Comet assay
  • Deguelin
  • DNA damage
  • DNA repair
  • Human lung cancer NCI-H460 cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Ji, B. C., Yu, C. C., Yang, S. U. T., Hsia, T. E. C., Yang, J. S., Lai, K. C., Ko, Y. C., Lin, J. J., Lai, T-I., & Chung, J. G. (2012). Induction of DNA damage by deguelin is mediated through reducing DNA repair genes in human non-small cell lung cancer NCI-H460 cells. Oncology Reports, 27(4), 959-964. https://doi.org/10.3892/or.2012.1622