Induction of differentiation in rat C6 glioma cells with saikosaponins

Yan J. Tsai; I. Ling Chen; Lin Y. Horng; Rong Tsun Wu

doi:10.1002/ptr.752

Induction of differentiation in rat C6 glioma cells with saikosaponins

Yan J. Tsai, I. Ling Chen, Lin Y. Horng, Rong Tsun Wu

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

The effects of saikosaponins (a, b₁, b₂, c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) were also noticeably increased after treatment with saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes.

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	Phytotherapy Research
Volume	16
Issue number	2
DOIs	https://doi.org/10.1002/ptr.752
Publication status	Published - 2002

Keywords

C6 glioma cells
Differentiation induction
Saikosaponins

ASJC Scopus subject areas

Organic Chemistry
Drug Discovery
Pharmacology

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10.1002/ptr.752

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abstract = "The effects of saikosaponins (a, b1, b2, c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) were also noticeably increased after treatment with saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes.",

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AU - Tsai, Yan J.

AU - Chen, I. Ling

AU - Horng, Lin Y.

AU - Wu, Rong Tsun

PY - 2002

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N2 - The effects of saikosaponins (a, b1, b2, c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) were also noticeably increased after treatment with saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes.

AB - The effects of saikosaponins (a, b1, b2, c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) were also noticeably increased after treatment with saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes.

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Induction of differentiation in rat C6 glioma cells with saikosaponins

Abstract

Keywords

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