Induction of cardiac fibrosis and transforming growth factor-β1 by motorcycle exhaust in rats

Ta-Liang Chen, Jiunn Wang Liao, Wei Hung Chan, Chiung Yuan Hsu, Jr Di Yang, Tzuu-Huei Ueng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Motorcycle exhaust (ME) is a major source of air pollution and a potential health hazard in urban areas where motorcycles are a popular means of transportation. The main objectives of this study were to determine the ability of ME to cause cardiotoxicity in rats and investigate the possible mechanisms of toxicity. Male rats were exposed to 1:10 diluted ME by inhalation 2 h daily and Monday through Friday for 8 weeks. Exposure to ME increased heart weight and decreased cardiac antioxidant enzymes glutathione S-transferase (GST), superoxide dismutase and glutathione peroxidase activities in a concentration-and time-dependent manner. Analysis of echocardiographic parameters indicated that ME increased left ventricle posterior wall thickness, interventricular septum thickness and left ventricle mass. Histopathological examinations of the hearts revealed that ME exposure caused focal cardial degeneration and necrosis, mononuclear cell infiltration, and fibrosis. The results of reverse transcriptase-polymerase chain reaction studies showed that ME decreased GST-M1 and GST-P1 mRNA expression and increased the expression of proinflammatory cytokine interleukin-1β, hypertrophy marker atrial natriuretic peptide, fibrosis markers type I and III collagen, profibrotic cytokine connective tissue growth factor, and hypertrophy and fibrosis mediator transforming growth factor (TGF)-β1 in the heart. The data of Western blot analysis showed that cardiac TGF-β1 protein was induced by ME. These findings demonstrate that subchronic ME exposure caused hypertrophy and fibrosis, and modulated GST and TGF-β1 expression in rat heart possibly by mechanisms involving oxidative stress and inflammation.

Original languageEnglish
Pages (from-to)525-535
Number of pages11
JournalInhalation Toxicology
Volume25
Issue number9
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Motorcycles
Transforming Growth Factors
Rats
Fibrosis
Glutathione Transferase
Hypertrophy
Heart Ventricles
Cytokines
Connective Tissue Growth Factor
Health hazards
Oxidative stress
Collagen Type III
Polymerase chain reaction
RNA-Directed DNA Polymerase
Air Pollution
Atrial Natriuretic Factor
Glutathione Peroxidase
Collagen Type I
Reverse Transcriptase Polymerase Chain Reaction
Air pollution

Keywords

  • Cardiotoxicity
  • Fibrosis
  • GST
  • Hypertrophy
  • Inflammation
  • Motorcycle exhaust
  • Oxidative stress
  • TGF-β1

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Induction of cardiac fibrosis and transforming growth factor-β1 by motorcycle exhaust in rats. / Chen, Ta-Liang; Liao, Jiunn Wang; Chan, Wei Hung; Hsu, Chiung Yuan; Yang, Jr Di; Ueng, Tzuu-Huei.

In: Inhalation Toxicology, Vol. 25, No. 9, 08.2013, p. 525-535.

Research output: Contribution to journalArticle

Chen, Ta-Liang ; Liao, Jiunn Wang ; Chan, Wei Hung ; Hsu, Chiung Yuan ; Yang, Jr Di ; Ueng, Tzuu-Huei. / Induction of cardiac fibrosis and transforming growth factor-β1 by motorcycle exhaust in rats. In: Inhalation Toxicology. 2013 ; Vol. 25, No. 9. pp. 525-535.
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