Induction of cardiac fibrosis and transforming growth factor-β1 by motorcycle exhaust in rats

Ta-Liang Chen, Jiunn Wang Liao, Wei Hung Chan, Chiung Yuan Hsu, Jr Di Yang, Tzuu-Huei Ueng

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Motorcycle exhaust (ME) is a major source of air pollution and a potential health hazard in urban areas where motorcycles are a popular means of transportation. The main objectives of this study were to determine the ability of ME to cause cardiotoxicity in rats and investigate the possible mechanisms of toxicity. Male rats were exposed to 1:10 diluted ME by inhalation 2 h daily and Monday through Friday for 8 weeks. Exposure to ME increased heart weight and decreased cardiac antioxidant enzymes glutathione S-transferase (GST), superoxide dismutase and glutathione peroxidase activities in a concentration-and time-dependent manner. Analysis of echocardiographic parameters indicated that ME increased left ventricle posterior wall thickness, interventricular septum thickness and left ventricle mass. Histopathological examinations of the hearts revealed that ME exposure caused focal cardial degeneration and necrosis, mononuclear cell infiltration, and fibrosis. The results of reverse transcriptase-polymerase chain reaction studies showed that ME decreased GST-M1 and GST-P1 mRNA expression and increased the expression of proinflammatory cytokine interleukin-1β, hypertrophy marker atrial natriuretic peptide, fibrosis markers type I and III collagen, profibrotic cytokine connective tissue growth factor, and hypertrophy and fibrosis mediator transforming growth factor (TGF)-β1 in the heart. The data of Western blot analysis showed that cardiac TGF-β1 protein was induced by ME. These findings demonstrate that subchronic ME exposure caused hypertrophy and fibrosis, and modulated GST and TGF-β1 expression in rat heart possibly by mechanisms involving oxidative stress and inflammation.

Original languageEnglish
Pages (from-to)525-535
Number of pages11
JournalInhalation Toxicology
Issue number9
Publication statusPublished - Aug 2013


  • Cardiotoxicity
  • Fibrosis
  • GST
  • Hypertrophy
  • Inflammation
  • Motorcycle exhaust
  • Oxidative stress
  • TGF-β1

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis


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