Induction of calcium release from isolated sarcoplasmic reticulum by triphenyltin

Jaw Jou Kang, I. Ling Chen, Yu Wen Cheng

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A direct peripheral myopathy has been found in organotin intoxication and suggested to be a significant factor in the development of muscle weakness following exposure. In this study, by using the isolated sarcoplasmic reticulum membrane vesicles, we have shown that triphenyltin dose-dependently induced Ca 2+ release from the actively and passively loaded sarcoplasmic reticulum vesicles. Triphenyltin induced Ca 2+ release in ruthenium red-sensitive and insensitive ways with EC 50 values of 75 and 270 μM, respectively. The Ca 2+-ATPase activity and Ca 2+ uptake of sarcoplasmic reticulum were also inhibited by triphenyltin. Triphenyltin exerted dual effects on the apparent [ 3H]ryanodine binding. Triphenyltin (0.5-10 μM) dose-dependently potentiated the [ 3H]ryanodine binding; however, the [ 3H] ryanodine binding decreased as the concentration of triphenyltin increased. The dissociation of bound [ 3H] ryanodine was facilitated by triphenyltin. The present study suggested that the internal Ca 2+ store of skeletal muscle could be depleted by triphenyltin through the inhibition of the Ca 2+ uptake and the induction of Ca 2+ release by acting on the Ca 2+-ATPase and Ca 2+ release channel, also known as the ryanodine receptor, of sarcoplasmic reticulum, respectively. These results could partly explain the development of muscle weakness in organotin intoxication; however, their relevance to the development of peripheral myopathy requires further examination.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalJournal of Biochemistry
Volume122
Issue number1
Publication statusPublished - Jul 1997
Externally publishedYes

Keywords

  • Ca release
  • Sarcoplasmic reticulum
  • Skeletal muscle
  • Triphenyltin

ASJC Scopus subject areas

  • Biochemistry

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