Induction of c-Fos immunoreactivity in the rat forebrain by conditioned and unconditioned aversive stimuli

Michael A. Pezzone, Wen Sen Lee, Gloria E. Hoffman, Bruce S. Rabin

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

The protein product of the c-fos proto-oncogene was immunocytochemically localized in forebrain regions of adult male Lewis rats subjected to a physically aversive footshock stimulus or a Pavlovian-conditioned, non-aversive, auditory stimulus. Animals receiving the conditioned stimulus were first conditioned by repeatedly pairing electric footshock, the unconditioned stimulus (US), with an auditory cue, the conditioned stimulus (CS). These animals were later tested with the CS in the absence of the US, a procedure which, like footshock itself, suppresses immune function. In animals exposed to the conditioned or unconditioned stressor, c-Fos was strongly expressed in cells of the paraventricular nuclei (PVN) of the hypothalamus, some of which contain corticotropin-releasing hormone (CRH), and other forebrain areas directly associated with autonomic function, the ventral lateal septal nuclei (LSV), the medial amygdaloid nuclei (AME), the sensorimotor cortex, the basal ganglia and thalamic nuclei. Control animals exhibited very little or no c-Fos in the above areas. The identified forebrain nuclei can now be targeted for further study aimed at elucidating their role in stress-induced immune alteration.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalBrain Research
Volume597
Issue number1
DOIs
Publication statusPublished - Nov 27 1992
Externally publishedYes

Fingerprint

Prosencephalon
Proto-Oncogene Proteins c-fos
Basal Ganglia
Thalamic Nuclei
Septal Nuclei
Paraventricular Hypothalamic Nucleus
Corticotropin-Releasing Hormone
Cell Nucleus
Hypothalamus
Cues

Keywords

  • c-Fos
  • Corticotropin-releasing hormone
  • Dorsomedial nucleus
  • Footshock
  • Lateral septal nucleus
  • Medial amygdaloid nucleus
  • Neuroimmunomodulation
  • Paraventricular nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Induction of c-Fos immunoreactivity in the rat forebrain by conditioned and unconditioned aversive stimuli. / Pezzone, Michael A.; Lee, Wen Sen; Hoffman, Gloria E.; Rabin, Bruce S.

In: Brain Research, Vol. 597, No. 1, 27.11.1992, p. 41-50.

Research output: Contribution to journalArticle

Pezzone, Michael A. ; Lee, Wen Sen ; Hoffman, Gloria E. ; Rabin, Bruce S. / Induction of c-Fos immunoreactivity in the rat forebrain by conditioned and unconditioned aversive stimuli. In: Brain Research. 1992 ; Vol. 597, No. 1. pp. 41-50.
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