Induction of apoptotic death by curcumin in human tongue squamous cell carcinoma SCC-4 cells is mediated through endoplasmic reticulum stress and mitochondria-dependent pathways

Siu Wan Ip, Shan Ying Wu, Chien Chih Yu, Chao Lin Kuo, Chun Shu Yu, Jai Sing Yang, Zen Pin Lin, Shang Ming Chiou, Hsiung Kwang Chung, Heng Chien Ho, Jing Gung Chung

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Curcumin from the rhizome of the Curcuma longa plant has been noted for its chemo-preventative and chemo-therapy activities, and it inhibits the growth of many types of human cancer cell lines. In this study, the mechanisms of cell death involved in curcumin-induced growth inhibition, including cell cycle arrest and induction of apoptosis in human tongue cancer SCC-4 cells, were investigated. Herein, we observed that curcumin inhibited cell growth of SCC-4 cells and induced cell death in a dose-dependent manner. Treatment of SCC-4 cells with curcumin caused a moderate and promoted the G 2/M phase arrest, which was accompanied with decreases in cyclin B/CDK1 and CDC25C protein levels. Moreover, curcumin significantly induced apoptosis of SCC-4 cells with a decrease of the Bcl-2 level, reduction of mitochondrial membrane potential (ΔΨ m), and promoted the active forms of caspase-3. Curcumin also promoted the releases of AIF and Endo G from the mitochondria in SCC-4 cells by using confocal laser microscope. Therefore, we suggest that curcumin induced apoptosis through a mitochondria-dependent pathway in SCC-4 cells. In addition, we also found that curcumin-induced apoptosis of SCC-4 cells was partly through endoplasmic reticulum stress. In conclusion, curcumin increased G 2/M phase arrest and induced apoptosis through ER stress and mitochondria-dependent pathways in SCC-4 cells.

Original languageEnglish
Pages (from-to)641-650
Number of pages10
JournalCell Biochemistry and Function
Volume29
Issue number8
DOIs
Publication statusPublished - Dec 2011

Keywords

  • Apoptosis
  • Curcumin
  • Endoplasmic reticulum stress
  • Human tongue cancer SCC-4 cells
  • Mitochondria

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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