Induction of apoptosis in human leukemic U937 cells by tetrandrine

Yuen Liang Lai, Yu Jen Chen, Tsu Yen Wu, Sheng Yuan Wang, Kuo Hua Chang, Chong Hung Chung, Mong Liang Chen

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Tetrandrine, a calcium channel antagonist, is a plant alkaloid possessing various pharmacological activities including anti-tumor activity. We studied tetrandrine to determine whether or not this anti-tumor effect occurs through induction of apoptosis. Tetrandrine inhibited both proliferation and clonogenicity of human leukemic U937 cells at an optimal concentration of 2.5 μg/ml. This growth inhibition was dose and time dependent, and accompanied with evidence of apoptotic changes. The characteristic morphological changes of apoptosis were observed in U937 cells under light microscopy and DNA fragmentation was noted by gel electrophoresis. Moreover, flow cytometric detection of surface phosphatidyl serine expression of U937 cells after treatment with tetrandrine confirmed the induction of apoptosis in these cells. The induction of apoptosis by tetrandrine would appear to proceed via non-Ca2+-dependent pathways.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalAnti-Cancer Drugs
Volume9
Issue number1
DOIs
Publication statusPublished - 1998

Keywords

  • Apoptosis
  • Leukemic cells
  • Tetrandrine

ASJC Scopus subject areas

  • Pharmacology
  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Induction of apoptosis in human leukemic U937 cells by tetrandrine'. Together they form a unique fingerprint.

  • Cite this

    Lai, Y. L., Chen, Y. J., Wu, T. Y., Wang, S. Y., Chang, K. H., Chung, C. H., & Chen, M. L. (1998). Induction of apoptosis in human leukemic U937 cells by tetrandrine. Anti-Cancer Drugs, 9(1), 77-81. https://doi.org/10.1097/00001813-199801000-00009