Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells

Jer Chia Tsai, Mukesh Jain, Chung Ming Hsieh, Wen Sen Lee, Masao Yoshizumi, Cam Patterson, Mark A. Perrella, Carol Cooke, Hong Wang, Edgar Haber, Robert Schlegel, Mu En Lee

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA strand breaks consistent with apoptosis. In addition, overexpression of Bcl-2 suppressed vascular smooth muscle cell death caused by PDTC and NAC. The viability of rat aortic smooth muscle cells decreased within 3 h of treatment with PDTC and was reduced to 30% at 12 h. The effect of PDTC and NAC on smooth muscle cells was not species specific because PDTC and NAC both caused dose- dependent reductions in viability in rat and human aortic smooth muscle cells. In contrast, neither PDTC nor NAC reduced viability in human aortic endothelial cells. The use of antioxidants to induce apoptosis in vascular smooth muscle cells may help prevent their proliferation in arteriosclerotic lesions.

Original languageEnglish
Pages (from-to)3667-3670
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number7
DOIs
Publication statusPublished - Feb 16 1996
Externally publishedYes

Fingerprint

Acetylcysteine
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Cells
Apoptosis
Rats
Endothelial cells
Endothelial Cells
Antioxidants
DNA Breaks
Lymphocytes
pyrrolidine dithiocarbamic acid
Cell death
Neurons
Chromatin
Condensation
Cell Death
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells. / Tsai, Jer Chia; Jain, Mukesh; Hsieh, Chung Ming; Lee, Wen Sen; Yoshizumi, Masao; Patterson, Cam; Perrella, Mark A.; Cooke, Carol; Wang, Hong; Haber, Edgar; Schlegel, Robert; Lee, Mu En.

In: Journal of Biological Chemistry, Vol. 271, No. 7, 16.02.1996, p. 3667-3670.

Research output: Contribution to journalArticle

Tsai, JC, Jain, M, Hsieh, CM, Lee, WS, Yoshizumi, M, Patterson, C, Perrella, MA, Cooke, C, Wang, H, Haber, E, Schlegel, R & Lee, ME 1996, 'Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells', Journal of Biological Chemistry, vol. 271, no. 7, pp. 3667-3670. https://doi.org/10.1074/jbc.271.7.3667
Tsai, Jer Chia ; Jain, Mukesh ; Hsieh, Chung Ming ; Lee, Wen Sen ; Yoshizumi, Masao ; Patterson, Cam ; Perrella, Mark A. ; Cooke, Carol ; Wang, Hong ; Haber, Edgar ; Schlegel, Robert ; Lee, Mu En. / Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 7. pp. 3667-3670.
@article{9cecd04a2eeb4f678c4680118eedf408,
title = "Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells",
abstract = "Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA strand breaks consistent with apoptosis. In addition, overexpression of Bcl-2 suppressed vascular smooth muscle cell death caused by PDTC and NAC. The viability of rat aortic smooth muscle cells decreased within 3 h of treatment with PDTC and was reduced to 30{\%} at 12 h. The effect of PDTC and NAC on smooth muscle cells was not species specific because PDTC and NAC both caused dose- dependent reductions in viability in rat and human aortic smooth muscle cells. In contrast, neither PDTC nor NAC reduced viability in human aortic endothelial cells. The use of antioxidants to induce apoptosis in vascular smooth muscle cells may help prevent their proliferation in arteriosclerotic lesions.",
author = "Tsai, {Jer Chia} and Mukesh Jain and Hsieh, {Chung Ming} and Lee, {Wen Sen} and Masao Yoshizumi and Cam Patterson and Perrella, {Mark A.} and Carol Cooke and Hong Wang and Edgar Haber and Robert Schlegel and Lee, {Mu En}",
year = "1996",
month = "2",
day = "16",
doi = "10.1074/jbc.271.7.3667",
language = "English",
volume = "271",
pages = "3667--3670",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "7",

}

TY - JOUR

T1 - Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells

AU - Tsai, Jer Chia

AU - Jain, Mukesh

AU - Hsieh, Chung Ming

AU - Lee, Wen Sen

AU - Yoshizumi, Masao

AU - Patterson, Cam

AU - Perrella, Mark A.

AU - Cooke, Carol

AU - Wang, Hong

AU - Haber, Edgar

AU - Schlegel, Robert

AU - Lee, Mu En

PY - 1996/2/16

Y1 - 1996/2/16

N2 - Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA strand breaks consistent with apoptosis. In addition, overexpression of Bcl-2 suppressed vascular smooth muscle cell death caused by PDTC and NAC. The viability of rat aortic smooth muscle cells decreased within 3 h of treatment with PDTC and was reduced to 30% at 12 h. The effect of PDTC and NAC on smooth muscle cells was not species specific because PDTC and NAC both caused dose- dependent reductions in viability in rat and human aortic smooth muscle cells. In contrast, neither PDTC nor NAC reduced viability in human aortic endothelial cells. The use of antioxidants to induce apoptosis in vascular smooth muscle cells may help prevent their proliferation in arteriosclerotic lesions.

AB - Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA strand breaks consistent with apoptosis. In addition, overexpression of Bcl-2 suppressed vascular smooth muscle cell death caused by PDTC and NAC. The viability of rat aortic smooth muscle cells decreased within 3 h of treatment with PDTC and was reduced to 30% at 12 h. The effect of PDTC and NAC on smooth muscle cells was not species specific because PDTC and NAC both caused dose- dependent reductions in viability in rat and human aortic smooth muscle cells. In contrast, neither PDTC nor NAC reduced viability in human aortic endothelial cells. The use of antioxidants to induce apoptosis in vascular smooth muscle cells may help prevent their proliferation in arteriosclerotic lesions.

UR - http://www.scopus.com/inward/record.url?scp=13344279414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13344279414&partnerID=8YFLogxK

U2 - 10.1074/jbc.271.7.3667

DO - 10.1074/jbc.271.7.3667

M3 - Article

C2 - 8631978

AN - SCOPUS:13344279414

VL - 271

SP - 3667

EP - 3670

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 7

ER -