Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells

Jer Chia Tsai, Mukesh Jain, Chung Ming Hsieh, Wen Sen Lee, Masao Yoshizumi, Cam Patterson, Mark A. Perrella, Carol Cooke, Hong Wang, Edgar Haber, Robert Schlegel, Mu En Lee

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Abstract

Pyrrolidinedithiocarbamate (PDTC) and N-acetylcysteine (NAC) have been used as antioxidants to prevent apoptosis in lymphocytes, neurons, and vascular endothelial cells. We report here that PDTC and NAC induce apoptosis in rat and human smooth muscle cells. In rat aortic smooth muscle cells, PDTC induced cell shrinkage, chromatin condensation, and DNA strand breaks consistent with apoptosis. In addition, overexpression of Bcl-2 suppressed vascular smooth muscle cell death caused by PDTC and NAC. The viability of rat aortic smooth muscle cells decreased within 3 h of treatment with PDTC and was reduced to 30% at 12 h. The effect of PDTC and NAC on smooth muscle cells was not species specific because PDTC and NAC both caused dose- dependent reductions in viability in rat and human aortic smooth muscle cells. In contrast, neither PDTC nor NAC reduced viability in human aortic endothelial cells. The use of antioxidants to induce apoptosis in vascular smooth muscle cells may help prevent their proliferation in arteriosclerotic lesions.

Original languageEnglish
Pages (from-to)3667-3670
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number7
DOIs
Publication statusPublished - Feb 16 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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  • Cite this

    Tsai, J. C., Jain, M., Hsieh, C. M., Lee, W. S., Yoshizumi, M., Patterson, C., Perrella, M. A., Cooke, C., Wang, H., Haber, E., Schlegel, R., & Lee, M. E. (1996). Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells. Journal of Biological Chemistry, 271(7), 3667-3670. https://doi.org/10.1074/jbc.271.7.3667