Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells

Ling Ling Yang, Chih Ying Lee, Kun Ying Yen

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Eugenia jambos L. (Myrtaceae) is an antipyretic and anti-inflammatory herb of Asian folk medicine. A 70% acetone extract exerted the strongest cytotoxic effects on human leukemia cells (HL-60) from a preliminary screening of 15 plants. The cytotoxic principles were separated by bio-assay-guided fractionation to HL-60 cells; two hydrolyzable tannins (1-O-galloyl castalagin and casuarinin) were isolated from the 70% acetone extract. All significantly inhibited human promyelocytic leukemia cell line HL-60 and showed less cytotoxicity to human adenocarcinoma cell line SK-HEP-1 and normal cell lines of human lymphocytes and Chang liver cells. Thus, these compounds were exhibited the dose-dependent manner in HL-60 cells and the IC50 were 10.8 and 12.5 μM, respectively. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G2/M phase, and a concomitant increase of cell population at G1 phase. The apoptosis induced by these two compounds was also demonstrated by DNA fragmentation assay and microscopic observation. These results suggest that the cytotoxic mechanism of both antitumor principle constituents might be the induction of apoptosis in HL-60 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)65-75
Number of pages11
JournalCancer Letters
Volume157
Issue number1
DOIs
Publication statusPublished - Aug 31 2000

Fingerprint

Hydrolyzable Tannins
Syzygium
HL-60 Cells
Leukemia
Apoptosis
Acetone
Cell Line
Myrtaceae
Antipyretics
G2 Phase
G1 Phase
Population Dynamics
Traditional Medicine
DNA Fragmentation
Cell Division
Inhibitory Concentration 50
Adenocarcinoma
Anti-Inflammatory Agents
Lymphocytes
Liver

Keywords

  • 1-O-galloyl castalagin
  • Apoptosis
  • Casuarinin
  • Cytotoxicity
  • Eugenia jambos
  • Human leukemia cells

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells. / Yang, Ling Ling; Lee, Chih Ying; Yen, Kun Ying.

In: Cancer Letters, Vol. 157, No. 1, 31.08.2000, p. 65-75.

Research output: Contribution to journalArticle

Yang, Ling Ling ; Lee, Chih Ying ; Yen, Kun Ying. / Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells. In: Cancer Letters. 2000 ; Vol. 157, No. 1. pp. 65-75.
@article{2e3be9da1ec04d23988ea0e919b27677,
title = "Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells",
abstract = "Eugenia jambos L. (Myrtaceae) is an antipyretic and anti-inflammatory herb of Asian folk medicine. A 70{\%} acetone extract exerted the strongest cytotoxic effects on human leukemia cells (HL-60) from a preliminary screening of 15 plants. The cytotoxic principles were separated by bio-assay-guided fractionation to HL-60 cells; two hydrolyzable tannins (1-O-galloyl castalagin and casuarinin) were isolated from the 70{\%} acetone extract. All significantly inhibited human promyelocytic leukemia cell line HL-60 and showed less cytotoxicity to human adenocarcinoma cell line SK-HEP-1 and normal cell lines of human lymphocytes and Chang liver cells. Thus, these compounds were exhibited the dose-dependent manner in HL-60 cells and the IC50 were 10.8 and 12.5 μM, respectively. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G2/M phase, and a concomitant increase of cell population at G1 phase. The apoptosis induced by these two compounds was also demonstrated by DNA fragmentation assay and microscopic observation. These results suggest that the cytotoxic mechanism of both antitumor principle constituents might be the induction of apoptosis in HL-60 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.",
keywords = "1-O-galloyl castalagin, Apoptosis, Casuarinin, Cytotoxicity, Eugenia jambos, Human leukemia cells",
author = "Yang, {Ling Ling} and Lee, {Chih Ying} and Yen, {Kun Ying}",
year = "2000",
month = "8",
day = "31",
doi = "10.1016/S0304-3835(00)00477-8",
language = "English",
volume = "157",
pages = "65--75",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells

AU - Yang, Ling Ling

AU - Lee, Chih Ying

AU - Yen, Kun Ying

PY - 2000/8/31

Y1 - 2000/8/31

N2 - Eugenia jambos L. (Myrtaceae) is an antipyretic and anti-inflammatory herb of Asian folk medicine. A 70% acetone extract exerted the strongest cytotoxic effects on human leukemia cells (HL-60) from a preliminary screening of 15 plants. The cytotoxic principles were separated by bio-assay-guided fractionation to HL-60 cells; two hydrolyzable tannins (1-O-galloyl castalagin and casuarinin) were isolated from the 70% acetone extract. All significantly inhibited human promyelocytic leukemia cell line HL-60 and showed less cytotoxicity to human adenocarcinoma cell line SK-HEP-1 and normal cell lines of human lymphocytes and Chang liver cells. Thus, these compounds were exhibited the dose-dependent manner in HL-60 cells and the IC50 were 10.8 and 12.5 μM, respectively. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G2/M phase, and a concomitant increase of cell population at G1 phase. The apoptosis induced by these two compounds was also demonstrated by DNA fragmentation assay and microscopic observation. These results suggest that the cytotoxic mechanism of both antitumor principle constituents might be the induction of apoptosis in HL-60 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - Eugenia jambos L. (Myrtaceae) is an antipyretic and anti-inflammatory herb of Asian folk medicine. A 70% acetone extract exerted the strongest cytotoxic effects on human leukemia cells (HL-60) from a preliminary screening of 15 plants. The cytotoxic principles were separated by bio-assay-guided fractionation to HL-60 cells; two hydrolyzable tannins (1-O-galloyl castalagin and casuarinin) were isolated from the 70% acetone extract. All significantly inhibited human promyelocytic leukemia cell line HL-60 and showed less cytotoxicity to human adenocarcinoma cell line SK-HEP-1 and normal cell lines of human lymphocytes and Chang liver cells. Thus, these compounds were exhibited the dose-dependent manner in HL-60 cells and the IC50 were 10.8 and 12.5 μM, respectively. Flow cytometric analysis demonstrated the presence of apoptotic cells with low DNA content, a decrease of cell population at G2/M phase, and a concomitant increase of cell population at G1 phase. The apoptosis induced by these two compounds was also demonstrated by DNA fragmentation assay and microscopic observation. These results suggest that the cytotoxic mechanism of both antitumor principle constituents might be the induction of apoptosis in HL-60 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - 1-O-galloyl castalagin

KW - Apoptosis

KW - Casuarinin

KW - Cytotoxicity

KW - Eugenia jambos

KW - Human leukemia cells

UR - http://www.scopus.com/inward/record.url?scp=0034739334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034739334&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(00)00477-8

DO - 10.1016/S0304-3835(00)00477-8

M3 - Article

C2 - 10893444

AN - SCOPUS:0034739334

VL - 157

SP - 65

EP - 75

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -