Induction of apoptosis and cell cycle arrest in glioma cells by GL331 (a topoisomerase II inhibitor)

Yun Chen, Yen Hao Su, Chih Hsien Wang, Jiann Ming Wu, Jin Cherng Chen, Sheng Hong Tseng

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Abstract

Background: GL331 is a topoisomerase II inhibitor and, in this study, the effects of GL331 upon rat C6 glioma cells were investigated. Materials and Methods: The glioma cells were treated with GL331, then a cytotoxicity assay was done to evaluate the cytotoxic effects of GL331 and flow-activated cell sorter analysis was performed to analyze the cellular apoptosis and cell cycle distribution of the glioma cells. The expressions of p53, p21 and 14-3-3σ in glioma cells treated with GL331 were investigated by Western blot analysis. Results: GL331 was demonstrated to exert cytotoxic effects upon and induce apoptosis of the C6 glioma cells in a concentration- and time-dependent manner (p < 0.05). GL331 also caused the glioma cells to accumulate in the G2/M-phase of the cell cycle and increased the expressions of p53, p21 and 14-3-3σ. Conclusion: GL331 exerts cytotoxic effects and induces apoptosis in C6 glioma cells, accompanied by cell accumulation in the G2/M-phase and activation of p53, p21 and 14-3-3σ.

Original languageEnglish
Pages (from-to)4203-4208
Number of pages6
JournalAnticancer Research
Volume25
Issue number6 B
Publication statusPublished - Nov 1 2005
Externally publishedYes

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Keywords

  • Apoptosis
  • Cell cycle
  • GL331
  • Glioma
  • Topoisomerase II inhibitor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Chen, Y., Su, Y. H., Wang, C. H., Wu, J. M., Chen, J. C., & Tseng, S. H. (2005). Induction of apoptosis and cell cycle arrest in glioma cells by GL331 (a topoisomerase II inhibitor). Anticancer Research, 25(6 B), 4203-4208.