Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6

Shih Jen Liu, Jy Ping Tsai, Chia Rui Shen, Yuh Pyng Sher, Chia Ling Hsieh, Yu Ching Yeh, Ai Hsiang Chou, Shu Rung Chang, Kuang Nan Hsiao, Feng Wei Yu, Hsin Wei Chen

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.

Original languageEnglish
Pages (from-to)354-360
Number of pages7
JournalJournal of Leukocyte Biology
Volume82
Issue number2
DOIs
Publication statusPublished - Aug 1 2007
Externally publishedYes

Fingerprint

Transforming Growth Factors
Interleukin-17
Interleukin-6
GATA3 Transcription Factor
Transcription Factors
Th17 Cells
Granzymes
Homeobox Genes
Tretinoin
Interleukin-10
Cell Differentiation
Up-Regulation
T-Lymphocytes
Phenotype
Messenger RNA

Keywords

  • Cytotoxic T lymphocyte
  • IL-17
  • Mixed lymphocyte culture

ASJC Scopus subject areas

  • Cell Biology

Cite this

Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6. / Liu, Shih Jen; Tsai, Jy Ping; Shen, Chia Rui; Sher, Yuh Pyng; Hsieh, Chia Ling; Yeh, Yu Ching; Chou, Ai Hsiang; Chang, Shu Rung; Hsiao, Kuang Nan; Yu, Feng Wei; Chen, Hsin Wei.

In: Journal of Leukocyte Biology, Vol. 82, No. 2, 01.08.2007, p. 354-360.

Research output: Contribution to journalArticle

Liu, SJ, Tsai, JP, Shen, CR, Sher, YP, Hsieh, CL, Yeh, YC, Chou, AH, Chang, SR, Hsiao, KN, Yu, FW & Chen, HW 2007, 'Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6', Journal of Leukocyte Biology, vol. 82, no. 2, pp. 354-360. https://doi.org/10.1189/jlb.0207111
Liu, Shih Jen ; Tsai, Jy Ping ; Shen, Chia Rui ; Sher, Yuh Pyng ; Hsieh, Chia Ling ; Yeh, Yu Ching ; Chou, Ai Hsiang ; Chang, Shu Rung ; Hsiao, Kuang Nan ; Yu, Feng Wei ; Chen, Hsin Wei. / Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6. In: Journal of Leukocyte Biology. 2007 ; Vol. 82, No. 2. pp. 354-360.
@article{e0bfe257887a4b1182d506d805fe5f0b,
title = "Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6",
abstract = "Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.",
keywords = "Cytotoxic T lymphocyte, IL-17, Mixed lymphocyte culture",
author = "Liu, {Shih Jen} and Tsai, {Jy Ping} and Shen, {Chia Rui} and Sher, {Yuh Pyng} and Hsieh, {Chia Ling} and Yeh, {Yu Ching} and Chou, {Ai Hsiang} and Chang, {Shu Rung} and Hsiao, {Kuang Nan} and Yu, {Feng Wei} and Chen, {Hsin Wei}",
year = "2007",
month = "8",
day = "1",
doi = "10.1189/jlb.0207111",
language = "English",
volume = "82",
pages = "354--360",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "FASEB",
number = "2",

}

TY - JOUR

T1 - Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6

AU - Liu, Shih Jen

AU - Tsai, Jy Ping

AU - Shen, Chia Rui

AU - Sher, Yuh Pyng

AU - Hsieh, Chia Ling

AU - Yeh, Yu Ching

AU - Chou, Ai Hsiang

AU - Chang, Shu Rung

AU - Hsiao, Kuang Nan

AU - Yu, Feng Wei

AU - Chen, Hsin Wei

PY - 2007/8/1

Y1 - 2007/8/1

N2 - Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.

AB - Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.

KW - Cytotoxic T lymphocyte

KW - IL-17

KW - Mixed lymphocyte culture

UR - http://www.scopus.com/inward/record.url?scp=34547769911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547769911&partnerID=8YFLogxK

U2 - 10.1189/jlb.0207111

DO - 10.1189/jlb.0207111

M3 - Article

VL - 82

SP - 354

EP - 360

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 2

ER -