Abstract
Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.
| Original language | English |
|---|---|
| Pages (from-to) | 354-360 |
| Number of pages | 7 |
| Journal | Journal of Leukocyte Biology |
| Volume | 82 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 1 2007 |
| Externally published | Yes |
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Keywords
- Cytotoxic T lymphocyte
- IL-17
- Mixed lymphocyte culture
ASJC Scopus subject areas
- Cell Biology
Cite this
Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6. / Liu, Shih Jen; Tsai, Jy Ping; Shen, Chia Rui; Sher, Yuh Pyng; Hsieh, Chia Ling; Yeh, Yu Ching; Chou, Ai Hsiang; Chang, Shu Rung; Hsiao, Kuang Nan; Yu, Feng Wei; Chen, Hsin Wei.
In: Journal of Leukocyte Biology, Vol. 82, No. 2, 01.08.2007, p. 354-360.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6
AU - Liu, Shih Jen
AU - Tsai, Jy Ping
AU - Shen, Chia Rui
AU - Sher, Yuh Pyng
AU - Hsieh, Chia Ling
AU - Yeh, Yu Ching
AU - Chou, Ai Hsiang
AU - Chang, Shu Rung
AU - Hsiao, Kuang Nan
AU - Yu, Feng Wei
AU - Chen, Hsin Wei
PY - 2007/8/1
Y1 - 2007/8/1
N2 - Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.
AB - Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.
KW - Cytotoxic T lymphocyte
KW - IL-17
KW - Mixed lymphocyte culture
UR - http://www.scopus.com/inward/record.url?scp=34547769911&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547769911&partnerID=8YFLogxK
U2 - 10.1189/jlb.0207111
DO - 10.1189/jlb.0207111
M3 - Article
VL - 82
SP - 354
EP - 360
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 2
ER -