Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6

Shih Jen Liu, Jy Ping Tsai, Chia Rui Shen, Yuh Pyng Sher, Chia Ling Hsieh, Yu Ching Yeh, Ai Hsiang Chou, Shu Rung Chang, Kuang Nan Hsiao, Feng Wei Yu, Hsin Wei Chen

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)


Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.

Original languageEnglish
Pages (from-to)354-360
Number of pages7
JournalJournal of Leukocyte Biology
Issue number2
Publication statusPublished - Aug 1 2007
Externally publishedYes


  • Cytotoxic T lymphocyte
  • IL-17
  • Mixed lymphocyte culture

ASJC Scopus subject areas

  • Cell Biology


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