Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6

Shih Jen Liu; Jy Ping Tsai; Chia Rui Shen; Yuh Pyng Sher; Chia Ling Hsieh; Yu Ching Yeh; Ai Hsiang Chou; Shu Rung Chang; Kuang Nan Hsiao; Feng Wei Yu; Hsin Wei Chen

doi:10.1189/jlb.0207111

Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6

Shih Jen Liu, Jy Ping Tsai, Chia Rui Shen, Yuh Pyng Sher, Chia Ling Hsieh, Yu Ching Yeh, Ai Hsiang Chou, Shu Rung Chang, Kuang Nan Hsiao, Feng Wei Yu, Hsin Wei Chen

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4⁺ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8⁺ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8⁺ cells exhibit a unique granzyme B^-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8⁺ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8⁺ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8⁺ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.

Original language	English
Pages (from-to)	354-360
Number of pages	7
Journal	Journal of Leukocyte Biology
Volume	82
Issue number	2
DOIs	https://doi.org/10.1189/jlb.0207111
Publication status	Published - Aug 1 2007
Externally published	Yes

Keywords

Cytotoxic T lymphocyte
IL-17
Mixed lymphocyte culture

ASJC Scopus subject areas

Cell Biology

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Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6. / Liu, Shih Jen; Tsai, Jy Ping; Shen, Chia Rui; Sher, Yuh Pyng; Hsieh, Chia Ling; Yeh, Yu Ching; Chou, Ai Hsiang; Chang, Shu Rung; Hsiao, Kuang Nan; Yu, Feng Wei; Chen, Hsin Wei.

In: Journal of Leukocyte Biology, Vol. 82, No. 2, 01.08.2007, p. 354-360.

Research output: Contribution to journal › Article › peer-review

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abstract = "Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that TGF-β and IL-6 could stimulate CD8+ cells to differentiate into noncytotoxic, IL-17-producing cells in MLC. These IL-17-producing CD8+ cells exhibit a unique granzyme B-IFN-γ-IL-10- phenotype. The mRNA level of Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 and Th1/Tc1 transcription factors T-box expressed in T cell (T-bet) as well as its target H2·O-like homeobox (Hlx) is decreased in CD8+ cells from TGF-β- and IL-6-treated MLC. In addition, these CD8+ cells display a marked up-regulation of retinoic acid-related orphan receptor-γt, a key IL-17 transcription factor. These results demonstrate that the existence of an IL-17-producing CD8+ subset belongs to neither the Tc1 nor the Tc2 subset and can be categorized as a T noncytotoxic 17 (Tnc17) subset.",

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Induction of a distinct CD8 Tnc17 subset by transforming growth factor-β and interleukin-6

Abstract

Keywords

ASJC Scopus subject areas

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