TY - JOUR
T1 - Induction of a decrease in renal NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activity by estradiol in rats
AU - Chang, Wen Chang
AU - Tai, Hsin Hsiung
N1 - Funding Information:
Acknow~edgemen&--Thaeu thorsw isht o thankM s. Sharon Parker for her excellent technicala ssistanceT. his work was supportedi n part by the American Heart Association Kentucky Affiliate, Inc., the University of Kentucky, Isabel1L . Kircher Fund, the NationalI nstituteso f Health (GM-30380), and the National Science Council of the Republic of China (NSC 73-0412-B006-06).
PY - 1985/6/15
Y1 - 1985/6/15
N2 - The effect of estradiol administration on renal and pulmonary NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activities in rats was studied. Estradiol induced a significant decrease in renal but not in pulmonary enzyme activity. Kinetic parameters for the renal enzyme from control and treated groups were compared. An identical apparent Km for prostaglandin E2 was obtained for the enzyme from both groups. Vmax in the treated group was progressively decreased and plateaued 1 day after estradiol injection. The estradiol-induced decrease in renal enzyme activity was blocked by an anti-estrogen, nafoxidine, suggesting that the effect of estradiol was a receptor-mediated event. The decrease in renal prostaglandin catabolic enzyme activity induced by estradiol may result in prolonging the half-life of circulating prostacyclin and may account, in part, for the anti-thrombogenic effect of estradiol.
AB - The effect of estradiol administration on renal and pulmonary NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activities in rats was studied. Estradiol induced a significant decrease in renal but not in pulmonary enzyme activity. Kinetic parameters for the renal enzyme from control and treated groups were compared. An identical apparent Km for prostaglandin E2 was obtained for the enzyme from both groups. Vmax in the treated group was progressively decreased and plateaued 1 day after estradiol injection. The estradiol-induced decrease in renal enzyme activity was blocked by an anti-estrogen, nafoxidine, suggesting that the effect of estradiol was a receptor-mediated event. The decrease in renal prostaglandin catabolic enzyme activity induced by estradiol may result in prolonging the half-life of circulating prostacyclin and may account, in part, for the anti-thrombogenic effect of estradiol.
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U2 - 10.1016/0006-2952(85)90397-1
DO - 10.1016/0006-2952(85)90397-1
M3 - Article
C2 - 4004926
AN - SCOPUS:0021808468
VL - 34
SP - 2073
EP - 2076
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 12
ER -