The effect of estradiol administration on renal and pulmonary NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activities in rats was studied. Estradiol induced a significant decrease in renal but not in pulmonary enzyme activity. Kinetic parameters for the renal enzyme from control and treated groups were compared. An identical apparent Km for prostaglandin E2 was obtained for the enzyme from both groups. Vmax in the treated group was progressively decreased and plateaued 1 day after estradiol injection. The estradiol-induced decrease in renal enzyme activity was blocked by an anti-estrogen, nafoxidine, suggesting that the effect of estradiol was a receptor-mediated event. The decrease in renal prostaglandin catabolic enzyme activity induced by estradiol may result in prolonging the half-life of circulating prostacyclin and may account, in part, for the anti-thrombogenic effect of estradiol.
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